E2F-1 overexpression correlates with decreased proliferation and better prognosis in adenocarcinomas of Barrett oesophagus
E2F-1 overexpression correlates with decreased proliferation and better prognosis in adenocarcinomas of Barrett oesophagus
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DOI：
10.1136/jcp.2007.050963
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发表时间：
2008-05-01
影响因子：
3.4
通讯作者：
Gorgoulis, V. G.
中科院分区：
文献类型：
--
作者：
Evangelou, K.;Kotsinas, A.;Gorgoulis, V. G.
背景：E2F-1 表达与食管鳞状细胞癌 (OSCC) 中的肿瘤生长呈正相关，而在结肠腺癌 (AC) 中表现出肿瘤抑制特征。迄今为止，尚无关于 Barrett 食管上发生的腺癌中 E2F-1 表达及其与肿瘤动力学（增殖、凋亡）关系的数据。 目的：由于食管腺癌几乎全部发生在化生 Barrett 上皮中，而相反的 E2F-1 行为似乎依赖于细胞和组织类型，因此我们研究了 E2F-1 在 Barrett 食管中的作用方式方法：我们评估了 35 个 Barrett 食管 AC 中 E2F-1、Ki-67、caspase-3 和 p53 的免疫组织化学表达状态。结果：通过半连续切片和统计分析，E2F-1 免疫阳性与 Ki-67 呈负相关（p = 0.023，Spearman 相关）。半连续切片分析揭示了 E2F-1 和 caspase-3 染色之间的直接关联。未发现与 p53 状态的相关性。 E2F-1免疫表达较高的病例表现出较长的生存期（p = 0.047，Cox回归）。结论：E2F-1表达与Barrett食管AC中的肿瘤增殖呈负相关。此外，E2F-1 免疫组织化学状态与患者生存呈正相关。这些发现与 OSCC 中的发现相反，表明食管腺癌中 E2F-1 的肿瘤抑制行为可能是由于化生 Barrett 粘膜的肠型性质所致。
Background: E2F-1 expression is positively associated with tumour growth in oesophageal squamous-cell carcinomas (OSCC), while it exhibits oncosuppressive features in colonic adenocarcinomas (AC). To date there are no data regarding E2F-1 expression and its relationship with tumour kinetics (proliferation, apoptosis) in adenocarcinomas that develop on Barrett oesophagus.Aim: As oesophageal adenocarcinomas occur almost exclusively in the metaplastic Barrett epithelium and the opposing E2F-1 behaviour seems to be cell and tissue-type dependent, we examined the manner in which E2F-1 acts in ACs of Barrett oesophagus.Methods: We estimated the immunohistochemical expression of E2F-1, Ki-67, caspase-3 and p53 immunohistochemical status in 35 Barrett oesophagus ACs.Results: E2F-1 immunopositivity correlated inversely with Ki-67, by semi-serial section and statistical analysis (p = 0.023, Spearman correlation). Semi-serial section analysis revealed a direct association between E2F-1 and caspase-3 staining. No correlation was found with p53 status. Cases with higher E2F-1 immunoexpression exhibited longer survival (p = 0.047, Cox-regression).Conclusions: E2F-1 expression was negatively related to tumour proliferation in ACs of Barrett oesophagus. Additionally, E2F-1 immunohistochemical status correlated positively with patient survival. These findings are opposite from those seen in OSCCs, suggesting that the tumour-suppressing E2F-1 behaviour in oesophageal adenocarcinomas is possibly due to the intestinal-type nature of the metaplastic Barrett mucosa.