Standardized Method for the Study of Antibody Neutralization of HCV Pseudoparticles (HCVpp)
Standardized Method for the Study of Antibody Neutralization of HCV Pseudoparticles (HCVpp)
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DOI：
10.1007/978-1-4939-8976-8_30
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发表时间：
2019-01-01
期刊：
影响因子：
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通讯作者：
Foung, Steven K. H.
中科院分区：
文献类型：
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作者：
Bailey, Justin R.;Urbanowicz, Richard A.;Foung, Steven K. H.
丙型肝炎病毒 (HCV) 假颗粒 (HCVpp) 是通过将 HCV 包膜（E1 和 E2）基因与逆转录病毒包装/报告基因构建体共转染到 HEK293T 细胞中而产生的。表面带有 HCV E1E2 蛋白的包膜颗粒通过逆转录病毒出芽过程释放到上清液中。病毒 E1E2 糖蛋白促进单轮受体介导的 HCVpp 进入肝癌细胞，这可以通过报告基因表达进行量化。这些 HCVpp 已用于研究 HCV 进入肝癌细胞的机制，以及免疫血清或 HCV 特异性单克隆抗体中和 HCV 的机制。
Hepatitis C virus (HCV) pseudoparticles (HCVpp) are generated by cotransfection of HCV envelope (E1 and E2) genes along with a retroviral packaging/reporter construct into HEK293T cells. Enveloped particles bearing HCV E1E2 proteins on their surface are released through a retroviral budding process into the supernatant. Viral E1E2 glycoproteins facilitate a single round of receptor-mediated entry of HCVpp into hepatoma cells, which can be quantified by reporter gene expression. These HCVpp have been employed to study mechanisms of HCV entry into hepatoma cells, as well as HCV neutralization by immune sera or HCV-specific monoclonal antibodies.