DNA and histone synthesis rate change during the S-period in Ehrlich ascites tumor cells.
DNA and histone synthesis rate change during the S-period in Ehrlich ascites tumor cells.
复制标题
艾利希腹水肿瘤细胞的 S 期 DNA 和组蛋白合成率发生变化。
作者：
Bloch,DP;Fu,CT;Dean,PN
流式细胞仪分析艾氏腹水癌细胞DNA的数据用非线性最小二乘曲线拟合程序进行拟合。对S时期合成速率的分析揭示了S时期DNA合成速率的变化规律。观察到三个主峰，其波谷至通过周期分别为0~16%，16~65%，65~100%。其间波谷的波峰速率和波谷速率之间的差异很小，约为最大波谷速率的10%，但这些波谷的波峰速率和波谷速率的差异是可重复的。在一天中的不同时间采集的样本之间，可以看到DNA分布特征的一些差异，因此可以看到速率特征的差异。它们被认为反映了昼夜节律的影响，但它们还不够大，不足以掩盖S时期发生的利率变化的一般模式。~3H-胸腺嘧啶核苷脉冲标记细胞在S时期的放射性分析与DNA分布的结果是一致的。对脱氧核糖核酸和组蛋白的平行分析表明，在S时期中期，两者的速率变化的时间和方向是一致的。
Data from flow-cytometric analysis of DNA of Ehrlich ascites tumor cells were fitted using non-linear least squares curve fitting routines. Analysis of rates of synthesis from the derived S-period profiles revealed a pattern of changing rates of DNA synthesis during the S-period. Three main peaks are seen whose trough to through periods range from 0 to 16%, 16 to 65%, 65 to 100% of the DNA synthesized during S. The differences between the peak rates and rates in the intervening troughs are small, about 10% of the maximum, but these occur reproducibly. Some differences in the DNA distribution profiles, hence rate profiles, can be seen among samples taken at different times during the day. These are thought to reflect the effects of circadian rhythms, but they are not large enough to obscure the general pattern of rate shifts that occur during the S-period. Analyses of radioactivity of3H-thymidine pulse labelled cells, sorted across the S-period, were in accord with the results obtained from the DNA distributions. A parallel analysis of DNA and histones showed a correspondence in the timing and direction of shifts in rate for both during the middle part of the S-period.