Culture of adult mouse neurons
Culture of adult mouse neurons
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DOI：
10.2144/05381rr02
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发表时间：
2005-01-01
期刊：
影响因子：
2.7
通讯作者：
McMurray, CT
中科院分区：
文献类型：
--
作者：
Eide, L;McMurray, CT
用于模拟生理的原代神经元细胞通常仅限于胚胎组织。然而，胚胎组织并不适合作为年龄相关生理变化或迟发性疾病的模型。然而，从历史上看，成功培养成年动物的神经元是困难的，如果不是不可能的话。在这里，我们报告了常规和可靠的培养成年小鼠健康纹状体神经元的方法。新方法具有成本效益，提高了神经元分离的速度和简单性。
Primary neuronal cells used to model physiology are generally limited to embryonic tissue. However embryonic tissue is not optimal as a model for age-related changes in physiology or late-onset disease. Successful culturing of neurons from adult animals, however has been historically difficult, if not impossible. Here, we report methodology for routine and reliable cultivation of healthy striatal neurons from adult mice. The new methodology is cost-effective and improves the speed and simplicity if neuronal isolation.