The PARP and VSG genes of Trypanosoma brucei do not resemble RNA polymerase II transcription units in sensitivity to Sarkosyl in nuclear run-on assays.
The PARP and VSG genes of Trypanosoma brucei do not resemble RNA polymerase II transcription units in sensitivity to Sarkosyl in nuclear run-on assays.
复制标题
在核连续检测中，布氏锥虫的 PARP 和 VSG 基因对肌氨酰的敏感性与 RNA 聚合酶 II 转录单位不同。
DOI：
10.1093/nar/20.2.303
复制
发表时间：
1992
影响因子：
14.9
通讯作者：
VanderPloeg,LH
中科院分区：
文献类型：
--
作者：
Rudenko,G;Lee,MG;VanderPloeg,LH
加入离子洗涤剂n- lauroylsarcos （Sarkosyl）影响原生动物锥虫bruceii基因的转录效率。添加萨科齐后，PARP（原环素或原环酸性重复蛋白）、变异细胞表面糖蛋白（VSG）和核糖体RNA （rRNA）基因的转录出现抗性或升高。相比之下，7个蛋白质编码house keeping基因和mini-exon donor RNA （medRNA）基因的转录被萨科奇齐的加入完全消除，而5S rRNA基因的转录则表现出中等敏感性。我们得出结论，萨科齐可用于区分不同类型的锥虫转录单位。PARP和VSG蛋白是基于它们对RNA聚合酶II抑制剂α-amanitin的抗性而被RNA聚合酶i样酶编码的。这种模式现在得到了他们对萨科齐加入的抵制的支持。
Addition of the ionic detergent N-lauroylsarcosine (Sarkosyl) affects the efficiency of transcription of genes of the protozoanTrypanosoma bruceiin nuclear ryn-on assays. Transcription of the PARP (procyclin or procyclic acidic repetitive protein), variant cell surface glycoprotein (VSG) and ribosomal RNA (rRNA) genes was resistant or increased after addition of Sarkosyl. In contrast, the transcription of seven protein coding house keeping genes and the mini-exon donor RNA (medRNA) genes was completely abolished by the addition of Sarkosyl, while the transcription of the 5S rRNA genes showed an intermediate sensitivity. We conclude that Sarkosyl can be used to discriminate between the different types of trypanosome transcription units. The PARP and VSG protein coding by an RNA polymerase I-like enzyme on the basis of their resistance to the RNA polymerase II inhibitor α-amanitin. This model is now supported by their resistance to the addition of Sarkosyl.