A novel whole-cell biocatalyst with NAD+ regeneration for production of chiral chemicals.
A novel whole-cell biocatalyst with NAD+ regeneration for production of chiral chemicals.
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DOI：
10.1371/journal.pone.0008860
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发表时间：
2010-01-26
期刊：
影响因子：
3.7
通讯作者：
Xu P
中科院分区：
文献类型：
--
作者：
Xiao Z;Lv C;Gao C;Qin J;Ma C;Liu Z;Liu P;Li L;Xu P
吡啶核苷酸辅助因子的高成本限制了NAD(P)依赖性氧化还原酶在工业规模上的应用。尽管NAD(P)H再生系统已被广泛研究，但在使用氧化形式的辅助因子的反应中需要的NAD(P)+再生，尚未得到很好的探索，特别是在全细胞生物催化过程中。研究了一种NAD+依赖酶和一种NAD+再生酶（短乳杆菌产生NADH氧化酶）在全细胞生物催化剂中的同时过表达，并将其应用于NAD+依赖氧化系统。采用以(2R,3R)-2,3-丁二醇脱氢酶为催化酶的全细胞生物催化剂制备了(3R)-乙托因、(3S)-乙托因和(2S,3S)-2,3-丁二醇。重组菌株共表达NAD+再生酶，在产手性乙酰素和(2S,3S)-2,3-丁二醇方面表现出显著提高的生物催化效率。通过使用不同的需要NAD(P)+催化的NAD(P)依赖性脱氢酶，可以将该共表达系统应用于其他手性化学物质的生产。
The high costs of pyridine nucleotide cofactors have limited the applications of NAD(P)-dependent oxidoreductases on an industrial scale. Although NAD(P)H regeneration systems have been widely studied, NAD(P)+ regeneration, which is required in reactions where the oxidized form of the cofactor is used, has been less well explored, particularly in whole-cell biocatalytic processes. Simultaneous overexpression of an NAD+ dependent enzyme and an NAD+ regenerating enzyme (H2O producing NADH oxidase from Lactobacillus brevis) in a whole-cell biocatalyst was studied for application in the NAD+-dependent oxidation system. The whole-cell biocatalyst with (2R,3R)-2,3-butanediol dehydrogenase as the catalyzing enzyme was used to produce (3R)-acetoin, (3S)-acetoin and (2S,3S)-2,3-butanediol. A recombinant strain, in which an NAD+ regeneration enzyme was coexpressed, displayed significantly higher biocatalytic efficiency in terms of the production of chiral acetoin and (2S,3S)-2,3-butanediol. The application of this coexpression system to the production of other chiral chemicals could be extended by using different NAD(P)-dependent dehydrogenases that require NAD(P)+ for catalysis.
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影响因子：
4.8
作者：
González, E;Fernández, MR;Biosca, JA
通讯作者：
Biosca, JA
DOI：
10.1007/b98911
发表时间：
2005-01-01
期刊：
TECHNOLOGY TRANSFER IN BIOTECHNOLOGY: FROM LAB TO INDUSTRY TO PRODUCTION
影响因子：
--
作者：
Wichmann, R;Vasic-Racki, D
通讯作者：
Vasic-Racki, D
影响因子：
56.9
作者：
Schoemaker, HE;Mink, D;Wubbolts, MG
通讯作者：
Wubbolts, MG
影响因子：
2.9
作者：
BRADFORD, MM
通讯作者：
BRADFORD, MM
影响因子：
5.4
作者：
Riebel, BR;Gibbs, PR;Bommarius, AS
通讯作者：
Bommarius, AS