POLAR LIPIDS OF GROUP-B STREPTOCOCCI .1. GLUCOSYLATED DIPHOSPHATIDYLGLYCEROL - NOVEL GLYCOPHOSPHOLIPID
POLAR LIPIDS OF GROUP-B STREPTOCOCCI .1. GLUCOSYLATED DIPHOSPHATIDYLGLYCEROL - NOVEL GLYCOPHOSPHOLIPID
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DOI：
10.1016/0005-2760(77)90045-5
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发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
FISCHER, W
中科院分区：
文献类型：
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作者：
FISCHER, W
从B组链球菌中分离出一种新的糖磷脂，它含有D-葡萄糖、甘油、酰基和P，其摩尔比为一比三比四比二。它被确定为2 ″-O-α。D-吡喃葡萄糖基-，1“，3”-双-（1，2-二酰基-sn-甘油-3-磷酸）-甘油。通过分析强碱性水解、史密斯降解和高碘酸盐氧化以及随后的肼解获得的分解产物，完成了脱酰化核心的结构。通过用磷脂酶A2和98%乙酸连续降解天然脂质来定位4个酰基。在B组中，25%的二磷脂酰甘油以其葡萄糖基化衍生物的形式存在，占脂质P的18%。未检测到葡萄糖基化磷脂酰甘油类似物。存在磷酸葡萄糖脂，初步鉴定为甘油磷酸二葡萄糖基二酰基甘油。
From group B streptococci a novel glycophospholipid was isolated, which contained D-glucose, glycerol, acyl groups and P in a molar ratio of .apprx. 1:3:4:2. It was established to be 2''-O-.alpha.-D-glucopyranosyl-, 1'',3''-bis-(1,2-diacyl-sn-glycero-3-phospho)-glycerol. The structure of the deacylated core was accomplished by analyses of the breakdown products obtained on strong alkaline hydrolysis, Smith-degradation and periodate oxidation with subsequent hydrazinolysis. The 4 acyl groups were located by sequential degradation of the native lipid with phospholipase A2 and 98% acetic acid. In group B streptococci .apprx. 25% of diphosphatidylglycerol occurs in the form of its glucosylated derivative which accounts for 18% of the lipid P. The glucosylated phosphatidylglycerol analog could not be detected. A phosphoglucolipid was present, which was tentatively identified as glycerophosphodiglucosyldiacylglycerol.