Patterns of auxin transport and gene expression during primordium development revealed by live imaging of the Arabidopsis inflorescence meristem
Patterns of auxin transport and gene expression during primordium development revealed by live imaging of the Arabidopsis inflorescence meristem
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DOI：
10.1016/j.cub.2005.09.052
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发表时间：
2005-11-08
期刊：
影响因子：
9.2
通讯作者：
Meyerowitz, EM
中科院分区：
文献类型：
--
作者：
Heisler, MG;Ohno, C;Meyerowitz, EM
背景：植物产生叶和花的原基来自一个特殊的组织，称为茎顶端分生组织（SAM）。遗传学研究已经确定了大量的基因，影响原基发育的各个方面，包括定位，生长和分化。然而，到目前为止，导致原基发展的事件的时空序列的详细了解还没有建立。结果如下：我们使用共聚焦成像的绿色荧光蛋白（GFP）报告基因在活植物中监测的多个蛋白质和基因的表达模式，参与花的原始发育过程。通过监测PINFORMED 1（PIN 1）的表达和极性，生长素流出的促进剂，和生长素反应的报告DR 5的表达，我们揭示了刻板的PIN 1极性变化，连同生长素诱导实验，表明生长素的建立和消耗周期伴随，并可能直接，原基发育的不同阶段。多个GFP-蛋白融合的成像显示，这些动态也与原始边界域，器官极性轴，和花分生组织起始的网站的规格。结论：这些结果提供了新的见解生长素运输动力学在原始定位和建议的作用，生长素运输影响原始细胞类型。
Background: Plants produce leaf and flower primordial from a specialized tissue called the shoot apical meristem (SAM). Genetic studies have identified a large number of genes that affect various aspects of primordium development including positioning, growth, and differentiation. So far, however, a detailed understanding of the spatio-temporal sequence of events leading to primordium development has not been established. Results: We use confocal imaging of green fluorescent protein (GFP) reporter genes in living plants to monitor the expression patterns of multiple proteins and genes involved in flower primordial developmental processes. By monitoring the expression and polarity of PINFORMED1 (PIN1), the auxin efflux facilitator, and the expression of the auxin-responsive reporter DR5, we reveal stereotypical PIN1 polarity changes which, together with auxin induction experiments, suggest that cycles of auxin build-up and depletion accompany, and may direct, different stages of primordium development. Imaging of multiple GFP-protein fusions shows that these dynamics also correlate with the specification of primordial boundary domains, organ polarity axes, and the sites of floral meristem initiation. Conclusions: These results provide new insight into auxin transport dynamics during primordial positioning and suggest a role for auxin transport in influencing primordial cell type.