Production of a Rat Monoclonal Antibody Against Brg1
Production of a Rat Monoclonal Antibody Against Brg1
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DOI：
10.1089/hyb.2009.0041
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发表时间：
2009-12-01
期刊：
影响因子：
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通讯作者：
Tachibana, Taro
中科院分区：
文献类型：
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作者：
Ohkawa, Yasuyuki;Harada, Akihito;Tachibana, Taro
Brm相关基因1（Brg 1）是SWI/SNF染色质重塑酶复合物的催化亚基，具有ATP酶活性。该复合物通过利用ATP水解的能量促进染色质重塑以用于基因表达。众所周知，SWI/SNF染色质重塑酶复合物对于细胞分化、细胞周期调节和胚胎发生是必需的。在这里，我们报告的建立杂交瘤细胞株产生的抗体Brg 1亚基的大鼠髂内淋巴结的方法。免疫印迹分析表明，我们的抗体可以特异性识别Brg 1。免疫细胞化学结果显示Brg 1定位于C2 C12成肌细胞核的常染色质。这些数据表明，该抗体是有用的分析Brg 1蛋白在细胞中的分子功能。
Brm-related gene-1 (Brg1) is a catalytic subunit of the SWI/SNF chromatin remodeling enzyme complex that has ATPase activity. This complex facilitates chromatin remodeling for gene expression by utilizing energy for ATP hydrolysis. It is well known that the SWI/SNF chromatin remodeling enzyme complex is essential for cell differentiation, cell cycle regulation, and embryogenesis. Here we report the establishment of a hybridoma cell line for producing an antibody against Brg1 subunit by the rat medial iliac lymph node method. Immunoblot analysis showed that our antibody can specifically recognize Brg1. It was revealed by immunocytochemistry that Brg1 is located in euchromatin of C2C12 myoblast nuclei. These data suggested this antibody is useful for analyzing molecular function of Brg1 protein in cells.