Quantitative analysis of the human ovarian carcinoma mitochondrial phosphoproteome
Quantitative analysis of the human ovarian carcinoma mitochondrial phosphoproteome
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DOI：
10.18632/aging.102199
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发表时间：
2019-08-31
期刊：
影响因子：
5.2
通讯作者：
Zhan, Xianquan
中科院分区：
文献类型：
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作者：
Li, Na;Qian, Shehua;Zhan, Xianquan
为探讨线粒体磷蛋白（mtPPs）在人卵巢癌（OC）中的存在及其潜在的生物学作用，采用基于TiO2富集的iTRAQ定量蛋白质组学方法，对卵巢癌组织和正常卵巢组织中纯化的线粒体进行分析。共鉴定出67个mtPPs，具有124个磷酸化位点，其中包括48个差异mtPPs（mtDPPs）。先前在OC中报告了18个mtPP，与先前的文献相比，本研究中的mtPP是一致的。GO分析显示这些线粒体PPs参与多种细胞过程。PPI网络显示这些mtPPs相互关联，其中一些mtPPs起着枢纽分子的作用，如EIF2S2、RPLPO、RPLP2、CFL 1、MYH 10、HSP 90、HSPD 1、PSMA 3、TMX 1、VDAC 2、VDAC 3、TOMM 22和TOMM 20。共检测到32个mtPP途径系统（p
To investigate the existence and their potential biological roles of mitochondrial phosphoproteins (mtPPs) in human ovarian carcinoma (OC), mitochondria purified from OC and control tissues were analyzed with TiO2 enrichment-based iTRAQ quantitative proteomics. Totally 67 mtPPs with 124 phosphorylation sites were identified, which of them included 48 differential mtPPs (mtDPPs). Eighteen mtPPs were reported previously in OCs, and they were consistent in this study compared to previous literature. GO analysis revealed those mtPPs were involved in multiple cellular processes. PPI network indicated that those mtPPs were correlated mutually, and some mtPPs acted as hub molecules, such as EIF2S2, RPLPO, RPLP2, CFL1, MYH10, HSP90, HSPD1, PSMA3, TMX1, VDAC2, VDAC3, TOMM22, and TOMM20. Totally 32 mtPP-pathway systems (p