The effect of iron limitation on expression of the aerobic and anaerobic electron transport pathway genes in Escherichia coli.
The effect of iron limitation on expression of the aerobic and anaerobic electron transport pathway genes in Escherichia coli.
复制标题
铁限制对大肠杆菌需氧和厌氧电子传递途径基因表达的影响。
DOI：
10.1111/j.1574-6968.1992.tb14045.x
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发表时间：
1992
影响因子：
2.1
通讯作者：
Gunsalus,RP
中科院分区：
文献类型：
--
作者：
Cotter,PA;Darie,S;Gunsalus,RP
为了确定大肠埃希氏菌的需氧和厌氧呼吸途径是否受铁供应的调节，用2，2‘-联吡啶作为螯合剂，在限制铁的培养基中培养了含有zacZ报告融合的yydAB，cyoABCDE，narGHJI，dmsABCandfrdABCD操纵子的菌株。在厌氧条件下，当铁限制时，厌氧呼吸途径操纵子Narg-lacZ、DMSA-lacZandfrdA-lacZ的表达减少了14-16倍。相比之下，有氧途径操纵子CyOA-lacZandcydA-LacZ的表达略有上升。对这些操纵子的铁依赖的转录控制不依赖于编码铁和DNA结合的调节蛋白的Furgene。Nr-lacZ的表达相对不受铁限制的影响，这表明细胞内的FNR水平不随铁的反应而改变。上述发现表明，除毛皮外，其他一些细胞蛋白可能与铁结合，以报告和调节铁依赖的细胞功能。
To determine whether the aerobic and anaerobic respiratory pathways ofEscherichia coliare regulated in response to iron availability, strains containinglacZreporter fusions to thecydAB,cyoABCDE, narGHJI, dmsABCandfrdABCDoperons were grown in medium limited for iron by use of the chelator, 2,2′-dipyridyl. Under anaerobic conditions, expression of the anaerobic respiratory pathway operons,narG-lacZ, dmsA-lacZandfrdA-lacZ, was reduced 14–16 fold when iron was limited. In contrast, expression of the aerobic pathway operons,cyoA-lacZandcydA-lacZ, was elevated modestly. Iron-dependent transcriptional control of these operons was independent of thefurgene which encodes an iron-and-DNA-binding regulatory protein. The expression offnr-lacZwas relatively unaffected by iron limitation suggesting that Fnr levels in the cell do not change in response to iron. The above findings suggest that in addition to Fur, some other cellular protein may bind iron for reporting and regulating iron-dependent cell functions.