THE ROLES OF SERUM AND CARBON DIOXIDE IN CAPSULE FORMATION BY BACILLUS ANTHRACIS.
THE ROLES OF SERUM AND CARBON DIOXIDE IN CAPSULE FORMATION BY BACILLUS ANTHRACIS.
复制标题
血清和二氧化碳在炭疽杆菌荚膜形成中的作用。
DOI：
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发表时间：
1964
期刊：
影响因子：
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通讯作者：
G. Meynell
中科院分区：
文献类型：
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作者：
E. Meynell;G. Meynell
摘要：已知炭疽杆菌强毒菌株在营养琼脂上形成荚膜取决于在空气中添加 CO2 的培养以及向培养基中添加血清或碳酸氢盐。 CO2 的最小有效浓度随介质 pH 值的变化而变化，这表明封装取决于介质中碳酸氢盐的阈值浓度。血清比碳酸氢盐更有效，并且似乎通过结合抑制胶囊形成的试剂来起作用，因为它可以被活性炭替代。该抑制剂可能是脂肪酸，因为某些酸阻止胶囊形成。仅当培养物变得非常致密后，才会在含有添加碳酸氢盐的营养琼脂上形成胶囊，这表明生物体要么使抑制剂失活，要么随着其生长速率在接近稳定期时下降而对其作用产生抗性。
SUMMARY: Capsule formation by virulent strains of Bacillus anthracis on nutrient agar is known to depend on incubation in air with added CO2 as well as the addition of serum or bicarbonate to the medium. The minimum effective concentration of CO2 varies with the pH of the medium in a way which shows that capsulation depends on a threshold concentration of bicarbonate in the medium. Serum is more effective than bicarbonate and appears to act by binding an agent which inhibits capsule formation since it is replaceable by activated charcoal. The inhibitor might be a fatty acid since certain acids prevented capsule formation. Capsules are formed on nutrient agar containing added bicarbonate only after the culture has become very dense which suggests that the organisms either inactivate the inhibitor or become resistant to its action as their growth rate falls on approaching the stationary phase.