Telomere length of normal leukocytes is affected by a functional polymorphism of hTERT
Telomere length of normal leukocytes is affected by a functional polymorphism of hTERT
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DOI：
10.1016/j.bbrc.2005.12.163
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发表时间：
2006-03-03
影响因子：
3.1
通讯作者：
Ikeda, Y
中科院分区：
文献类型：
--
作者：
Matsubara, Y;Murata, M;Ikeda, Y
人端粒酶逆转录酶（human telomerase reverse transcriptase，hTERT）是端粒酶的催化亚基，其转录调控是端粒酶活性与端粒长度相关的关键。在这项研究中，我们调查了一个T-1327/C多态性的hTERT启动子区域内的hTERT启动子活性和白细胞端粒长度在正常人的影响。T-1327-序列中的启动子活性显著高于C-1327-序列中的启动子活性（p = 0.0004）。对于白细胞端粒长度，T-1327等位基因携带者比T-1327等位基因非携带者显著更长（p = 0.0007）。此外，T-1327/T（p = 0.6633）和T-1327/C受试者（p = 0.1691）的白细胞端粒长度没有出现与年龄相关的缩短，而C-1327/C受试者中出现明显的与年龄相关的端粒缩短（p = 0.0117）。这些发现表明，在正常个体中，hTERT的功能性T-1327/C多态性与白细胞端粒长度相关。(c)2006年爱思唯尔公司All rights reserved.
Transcriptional regulation of human telomerase reverse transcriptase (hTERT), a catalytic subunit of telomerase, is essential for telomerase activity associated with telomere length. In this study, we investigated the effects of a T-1327/C polymorphism within the hTERT promoter region on the hTERT promoter activity and leukocyte telomere length in normal individuals. The promoter activity in the T-1327-sequence was significantly higher than that in the C-1327-sequence (p = 0.0004). For leukocyte telomere length, the T-1327-allele carriers had significantly longer than the T-1327-allele non-carriers (p = 0.0007). Also, there was no age-related shortening in leukocyte telomere length in the T-1327/T (p = 0.6633) and T-1327/C subjects (p = 0.1691), whereas there was clear age-related telomere shortening in the C-1327/C subjects (p = 0.0117). These findings suggest that the functional T-1327/C polymorphism of hTERT is associated with leukocyte telomere length in normal individuals. (c) 2006 Elsevier Inc. All rights reserved.