First order dissociation rates between a subpopulation of high affinity rabbit anti-fluorescyl IgG antibody and homologous ligand.
First order dissociation rates between a subpopulation of high affinity rabbit anti-fluorescyl IgG antibody and homologous ligand.
复制标题
高亲和力兔抗荧光 IgG 抗体亚群与同源配体之间的一级解离率。
DOI：
10.1016/0161-5890(80)90020-6
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发表时间：
1980
影响因子：
3.6
通讯作者：
E. Voss
中科院分区：
文献类型：
--
作者：
R. M. Watt;J. Herron;E. Voss
用Green（1963）的方法测定了纯化的高免疫兔抗荧光素IgG抗体的配体亚群的一级解离率。在过量未标记的同源半抗原存在的情况下，测量了放射性标记荧光素配体的解离。三种不同抗体制备的结果表明，配体亚群的解离率分别为1.3 × 10[su−3,2.4 × 10−4和2.2 × 10−6sec−1。假设关联速率为4.6 × 108M−1sec−1(Levisonet al., 1971)，计算出的平均平衡常数范围为3.1 × 1011M−1至2.1 × 1014M−1。这些值是报道的抗体-半抗原相互作用的最高值。
The first order dissociation rates of liganded subpopulations of purified hyperimmune rabbit antifluorescyl IgG antibodies were determined using the method of Green (1963). The dissociation of radiolabeled fluorescyl ligands was measured in the presence of excess unlabeled homologous hapten. Results with three different antibody preparations indicated dissociation rates of 1.3 × 10[su−3, 2.4 × 10−4and 2.2 × 10−6sec−1for the liganded subpopulations. Assuming an association rate of 4.6 × 108M−1sec−1(Levisonet al., 1971), average equilibrium constants ranging from 3.1 × 1011M−1to 2.1 × 1014M−1were calculated. These values are among the highest reported for antibody-hapten interactions.