The effects of ionizing radiation on osteoblast-like cells in vitro
The effects of ionizing radiation on osteoblast-like cells in vitro
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DOI：
10.1097/00006534-200010000-00015
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发表时间：
2000-10-01
影响因子：
3.6
通讯作者：
Longaker, MT
中科院分区：
文献类型：
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作者：
Dudziak, ME;Saadeh, PB;Longaker, MT
众所周知，电离辐射对骨折部位骨再生的不利影响包括骨细胞数量减少，成骨细胞活性受到抑制，血管减少。然而，放射性骨坏死和照射后骨折愈合受损的生物学机制仍不清楚。电离辐射可能通过改变成骨细胞分化状态引起或导致的细胞因子表达谱而降低成功的骨修复。这些变化反过来可能导致成骨细胞增殖和细胞外基质形成的变化。本研究旨在探讨电离辐射对体外培养的MC3T3-E1成骨样细胞增殖、成熟及细胞因子产生的影响。具体地说，作者研究了不同剂量的电离辐射(0、40、400和800cGy)对转化生长因子-β1(转化生长因子-β1)、血管内皮生长因子和碱性磷酸酶表达的影响。此外，作者还研究了电离辐射对MC3T3-E1细胞增殖的影响以及从对照细胞和照射细胞获得的条件培养液对牛主动脉内皮细胞增殖的调节能力。最后，作者评估腺病毒介导的转化生长因子-β-1基因治疗的效果，以努力“拯救”受辐射的成骨细胞。
The well-described detrimental effects of ionizing radiation on the regeneration of bone within a fracture site include decreased osteocyte number, suppressed osteoblast activity, and diminished vascularity. However, the biologic mechanisms underlying osteoradionecrosis and the impaired fracture healing of irradiated bone remain undefined. Ionizing radiation may decrease successful osseous repair by altering cytokine expression profiles resulting from or leading to a change in the osteoblastic differentiation state. These changes may, in turn, cause alterations in osteoblast proliferation and extracellular matrix formation. The purpose of this study was to investigate the effects of ionizing radiation on the proliferation, maturation, and cytokine production of MC3T3-E1 osteoblast-like cells in vitro. Specifically, the authors examined the effects of varying doses of ionizing radiation (0, 40, 400, and 800 cGy) on the expression of transforming growth factor-β1 (TGF-β1), vascular endothelial growth factor (VEGF), and alkaline phosphatase. In addition, the authors studied the effects of ionizing radiation on MC3T3-E1 cellular proliferation and the ability of conditioned media obtained from control and irradiated cells to regulate the proliferation of bovine aortic endothelial cells. Finally, the authors evaluated the effects of adenovirus-mediated TGF-β1 gene therapy in an effort to “rescue” irradiated osteoblasts.