Dopaminergic transmission in the rat retina: Evidence for volume transmission
Dopaminergic transmission in the rat retina: Evidence for volume transmission
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DOI：
10.1016/s0891-0618(96)00176-7
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发表时间：
1996-11-01
影响因子：
2.8
通讯作者：
Fuxe, K
中科院分区：
文献类型：
--
作者：
Bjelke, B;Goldstein, M;Fuxe, K
该研究旨在确定视网膜汽车中的多巴胺能神经传递是否通过体积传输进行操作。在双重免疫标记实验中，大鼠视网膜中酪氨酸羟化酶 (TH) 和多巴胺 (DA) 免疫反应性 (ir) 末端和细胞体与多巴胺 D2 受体样 ir 细胞体和过程之间存在不匹配和匹配。匹配区域位于内核和丛状层（D2 ir 细胞体加突起）。错配区域位于神经节细胞层、外丛状层和感光层外段，其中与 D2 类似 ir 过程相关的 TH ir 末端很少。在分析 D1 受体样 ir 突与 TH ir 神经末梢的类似实验中，主要可以证明它们分布的不匹配，其中 D1 样 ir 突存在于外丛状层和外节中，其中也存在 D2 样受体的不匹配。 TH 末端丛的定位与外丛状层、外节和神经节细胞层中的多巴胺 D2 和 D1 受体亚型（仅 D2 免疫反应性（IR））之间的不匹配表明，主要来自内丛状层的多巴胺可能通过细胞外空间中的扩散到达 D2 和 D1 错配受体。将多巴胺注入玻璃体后，多巴胺通过视网膜扩散，整个内丛状层和整个外丛状层出现强烈的儿茶酚胺（CA）荧光，分别代表匹配和不匹配的DA受体区域。 DA可能与两个丛状层中的D1和D2受体结合，因为DA受体拮抗剂氯丙嗪完全阻断DA荧光的出现，而氟哌啶醇处理后仅发现部分阻断，主要阻断D2受体。这些结果表明，在外丛状层中具有稀疏分支的无长突和/或丛状间 DA 细胞可以通过大鼠视网膜中的体积传输来影响外丛状层和外节，以及大鼠视网膜的其他层，例如神经节细胞层。版权所有 (C) 1996 Elsevier Science B.V.
The study was designed to determine whether dopaminergic neurotransmission in the retina car, operate via volume transmission. In double immunolabelling experiments, a mismatch as well as a match was demonstrated in the rat retina between tyrosine hydroxylase (TH) and dopamine (DA) immunoreactive (ir) terminals and cell bodies and dopamine D2 receptor-like ir cell bodies and processes. The match regions were located in the inner nuclear and plexiform layers (D2 ir cell bodies plus processes). The mismatch regions were located in the ganglion cell layer, the outer plexiform layer, and the outer segment of the photoreceptor layer, where very few TH ir terminals can be found in relation to the D2 like ir processes. In similar experiments analyzing D1 receptor like ir processes versus TH ir nerve terminals, mainly a mismatch in their distribution could be demonstrated, with the D1 like ir processes present in the outer plexiform layer and the outer segment where a mismatch in D2 like receptors also exists. The demonstration of a mismatch between the localization of the TH terminal plexus and the dopamine D2 and D1 receptor subtypes in the outer plexiform layer, the outer segment and the ganglion cell layer (only D2 immunoreactivity (IR)) suggests that dopamine, mainly from the inner plexiform layer, may reach the D2 and D1 mismatch receptors via diffusion in the extracellular space. After injecting dopamine into the corpus vitreum, dopamine diffuses through the retina, and strong catecholamine (CA) fluorescence appears in the entire inner plexiform layer and the entire outer plexiform layer, representing the match and mismatch DA receptor areas, respectively. The DA is probably bound to D1 and D2 receptors in both plexiform layers, since the DA receptor antagonist chlorpromazine fully blocks the appearance of the DA fluorescence, while only a partial blockade is found after haloperidol treatment which mainly blocks D2 receptors. These results indicate that the amacrine and/or interplexiform DA cells, with sparse branches in the outer plexiform layer, can operate via volume transmission in the rat retina to influence the outer plexiform layer and the outer segment, as well as other layers of the rat retina such as the ganglion cell layer. Copyright (C) 1996 Elsevier Science B.V.