Prokaryotic promoters in biotechnology.
Prokaryotic promoters in biotechnology.
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DOI：
10.1016/s1387-2656(08)70049-8
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发表时间：
1995-01-01
期刊：
影响因子：
--
通讯作者：
Doi, R H
中科院分区：
文献类型：
--
作者：
Goldstein, M A;Doi, R H
描述了原核启动子的基本性质和启动子区域，重点介绍了在大肠杆菌和枯草芽孢杆菌中发现的启动子。比较主要形式和次要形式的RNA聚合酶全酶识别的启动子的特异性和差异。为了有效和受调控的转录而构建的自然启动子和杂交启动子都从它们的用途方面进行了讨论。由于启动子区域包含不仅可被RNA聚合酶识别的序列，还可被调节启动子表达的正负调控因子识别的序列，因此还描述了这些启动子区域的功能和特性。讨论了原核启动子在表达外源基因用于生物技术方面的应用现状和前景。
The basic properties of prokaryotic promoters and the promotor region are described with special emphasis on promoters that are found in Escherichia coli and Bacillus subtilis. Promoters recognized by major and minor forms of RNA polymerase holoenzymes are compared for their specificities and differences. Both natural and hybrid promoters that have been constructed for purposes of efficient and regulated transcription are discussed in terms of their utility. Since promoter regions contain sequences that are recognized not only by RNA polymerase but by positive and negative regulatory factors that regulate expression from promoters, the functions and properties of these promoter regions are also described. The current utility and the future prospects of the prokaryotic promoters in expressing heterologous genes for biotechnology purposes are discussed.