Patterns of soybean proline-rich protein gene expression.
Patterns of soybean proline-rich protein gene expression.
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DOI：
10.1105/tpc.4.1.99
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发表时间：
1992-01
期刊：
影响因子：
--
通讯作者：
R. Wyatt;R. T. Nagao;J. L. Key
中科院分区：
文献类型：
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作者：
R. Wyatt;R. T. Nagao;J. L. Key
利用原位杂交实验研究了大豆富含脯氨酸蛋白（SbPRPs）基因家族的三个成员的表达模式。在大多数情况下，SbPRP基因的表达在特定器官的有限数量的细胞类型中是强烈的。SbPRP1 RNA定位于大豆下胚轴的几种细胞类型，包括韧皮部和木质部内的细胞。SbPRP1的表达增加表皮细胞中的伸长和成熟地区的下胚轴，也检测到在成熟种子的种脐周围的木质部细胞的表达。SbPRP2 RNA存在于皮层细胞和下胚轴的维管组织中，尤其是韧皮部细胞。该基因也在成熟种皮的内珠被中表达。SbPRP3 RNA特异性地定位于下胚轴伸长区中柱周围细胞的内胚层，以及叶片和子叶的表皮细胞中。这些数据表明，该基因家族的成员表现出细胞特异性表达。SbPRP基因家族的成员在不同类型的细胞中表达，并且在某些细胞类型中也表达富含甘氨酸的蛋白质或富含羟脯氨酸的糖蛋白类基因。
The expression patterns of three members of a gene family that encodes proline-rich proteins in soybean (SbPRPs) were examined using in situ hybridization experiments. In most instances, the expression of SbPRP genes was intense in a limited number of cell types of a particular organ. SbPRP1 RNA was localized in several cell types of soybean hypocotyls, including cells within the phloem and xylem. SbPRP1 expression increased within epidermal cells in the elongating and mature regions of the hypocotyl; expression was detected also in lignified cells surrounding the hilum of mature seeds. SbPRP2 RNA was present in cortical cells and in the vascular tissue of the hypocotyl, especially cells of the phloem. This gene was expressed also in the inner integuments of the mature seed coat. SbPRP3 RNA was localized specifically to the endodermoid layer of cells surrounding the stele in the elongating region of the hypocotyl, as well as in the epidermal cells of leaves and cotyledons. These data show that members of this gene family exhibit cell-specific expression. The members of the SbPRP gene family are expressed in different types of cells and in some cell types that also express the glycine-rich protein or hydroxyproline-rich glycoprotein classes of genes.