Lymphocyte activation and subset redistribution in the peripheral blood in acute malaria illness: Distinct gamma delta(+) T cell patterns in Plasmodium falciparum and P-vivax infections
Lymphocyte activation and subset redistribution in the peripheral blood in acute malaria illness: Distinct gamma delta(+) T cell patterns in Plasmodium falciparum and P-vivax infections
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DOI：
10.1046/j.1365-2249.1997.d01-981.x
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发表时间：
1997-04-01
影响因子：
4.6
通讯作者：
Christensson, B
中科院分区：
文献类型：
--
作者：
Worku, S;Bjorkman, A;Christensson, B
对来自埃塞俄比亚阿迪斯和拿撒勒的39名急性疟疾患者和16名健康对照者外周血淋巴细胞亚群分布和活化进行了研究。经聚合酶链反应（PCR）证实，15例患者感染恶性疟原虫（Pf），17例感染间日疟原虫（Pv），7例为Pf和Pv双重感染（Di）。三色流式细胞术用于表型分型。疟疾患者的总白细胞和淋巴细胞计数低于对照组。Pf患者的T细胞计数减少，而Pv和Di患者的自然杀伤（NK）细胞计数减少。CD 4/CD 8比值保持不变。Pf和Di患者中γ δ（+）T细胞显著升高，但Pv患者中没有。γ δ（+）T细胞的增加主要是由于V δ 1（+）细胞的增加。通过对CD 25和HLA-DR表达的细胞活化分析显示，与对照组相比，所有患者组中活化的CD 3（+）细胞（包括γ δ（+）T细胞）的数量均显著增加。因此，我们的研究结果表明，在急性疟疾疾病中，淋巴细胞亚群分布和活化（包括γ δ（+）T细胞）存在复杂的变化模式。Pf感染的这些模式似乎与Pv感染的模式不同。
Lymphocyte subset distributions and activation in the peripheral blood were studied in 39 patients with acute malaria and 16 healthy controls from Addis Ababa and Nazareth, Ethiopia. As confirmed by polymerase chain reaction (PCR), 15 patients were infected with Plasmodium falciparum (Pf), 17 with P. vivax (Pv) and seven were double-infected (Di) with both Pf and Pv. Three-colour flow cytometry was used for phenotyping. Total leucocyte and lymphocyte counts were lower in malaria patients than in controls. The T cell count was reduced in Pf patients, while in the Pv and Di patients there was a reduction in the natural killer (NK) cell count. The CD4/CD8 ratio remained unchanged. gamma delta(+) T cells were significantly elevated in Pf and Di patients, but not in Pv patients. The increase in gamma delta(+) T cells was mostly due to an increase in V delta 1(+) cells. Analyses of cellular activation indicated by the expressions of CD25 and HLA-DR revealed significantly higher numbers of activated CD3(+) cells, including gamma delta(+) T cells, in all patient groups compared with controls. Our results thus indicate that in acute malaria illness there is a complex pattern of change in lymphocyte subset distribution and activation, including gamma delta(+) T cells. These patterns in Pf infection seem to be distinct from those in Pv infection.