Distinct contributions from the hindbrain and mesenchyme to inner ear morphogenesis
Distinct contributions from the hindbrain and mesenchyme to inner ear morphogenesis
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DOI：
10.1016/j.ydbio.2009.11.001
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发表时间：
2010-01-15
影响因子：
2.7
通讯作者：
Wu, Doris K.
中科院分区：
文献类型：
--
作者：
Liang, Jennifer K.;Bok, Jinwoong;Wu, Doris K.
一个成熟的内耳是一个复杂的结构，包括前庭和听觉组件。在卵内进行显微外科消融、旋转和移位，以确定控制内耳形态发生的组织。我们发现，间充质/外胚层相邻的发展中的耳朵专门管理前庭组件的形状-半规管和壶腹-通过赋予这些结构的前后轴向信息。与此相反，删除个别后脑菱球相邻的发展中的耳朵优先影响的听觉细分，耳蜗管，或基底乳头的生长和形态发生。去除第5菱形节影响耳蜗管的生长，而去除第6菱形节影响耳蜗的生长和形态发生。沿前后轴沿着旋转菱体5和6也影响耳蜗管形态发生，但对前庭成分影响不大。我们的研究表明，离散的组织，在一定距离上起作用。控制内耳不同部分的形态发生。这些结果为确定脊椎动物前庭和听觉发育所必需的因素提供了基础。(C)2009年由Elsevier Inc.出版
A mature inner ear is a complex structure consisting of vestibular and auditory components. Microsurgical ablations, rotations, and translocations were performed in ovo to identify the tissues that control inner ear morphogenesis. We show that mesenchyme/ectoderm adjacent to the developing ear specifically governs the shape of vestibular components - the semicircular canals and ampullae - by conferring anteroposterior axial information to these structures. In contrast, removal of individual hindbrain rhombomeres adjacent to the developing ear preferentially affects the growth and morphogenesis of the auditory subdivision, the cochlear duct, or basilar papilla. Removal of rhombomere 5 affects cochlear duct growth, while rhombomere 6 removal affects cochlear growth and morphogenesis. Rotating rhombomeres 5 and 6 along the anteroposterior axis also impacts cochlear duct morphogenesis but has little effect on the vestibular components. Our studies indicate that discrete tissues, acting at a distance. control the morphogenesis of distinct elements of the inner ear. These results provide a basis for identifying factors that are essential to vestibular and auditory development in vertebrates. (C) 2009 Published by Elsevier Inc.