Defined DNA/nanoparticle conjugates
Defined DNA/nanoparticle conjugates
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DOI：
10.1073/pnas.0506290102
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发表时间：
2005-09-20
影响因子：
11.1
通讯作者：
Kornberg, RD
中科院分区：
文献类型：
--
作者：
Ackerson, CJ;Sykes, MT;Kornberg, RD
通过与19或20个残基的硫醇化的硫代氨基甲酸酯进行位置交换，使葡聚糖单分子层保护的金簇反应。所得的DNA/纳米颗粒缀合物可以通过凝胶电泳基于结合的寡核苷酸的数量分离，并通过DNA-DNA杂交彼此组装。这种方法克服了DNA/纳米颗粒合成的先前限制，并产生相对于金和核酸含量精确定义的缀合物。
Glutathione monolayer-protected gold clusters were reacted by place exchange with 19 or 20-residue thiolated oligonucleoticles. The resulting DNA/nanoparticle conjugates could be separated on the basis of the number of bound oligonucleoticles by gel electrophoresis and assembled with one another by DNA-DNA hybridization. This approach overcomes previous limitations of DNA/nanoparticle synthesis and yields conjugates that are precisely defined with respect to both gold and nucleic acid content.