Internal pH indicators for biomolecular NMR
Internal pH indicators for biomolecular NMR
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DOI：
10.1007/s10858-008-9234-6
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发表时间：
2008-05-01
影响因子：
2.7
通讯作者：
Sykes, Brian D.
中科院分区：
文献类型：
--
作者：
Baryshnikova, Olga K.;Williams, Thomas C.;Sykes, Brian D.
我们描述了一种非侵入性的技术，用于确定pH值的生物分子NMR样品使用缓冲液成分（甲酸，三，哌嗪，咪唑）作为内部pH指示剂，其H-1 NMR化学位移是敏感的pH值范围从2.5到9.8。该方法适用于在NMR滴定或结构基因组学或代谢组学的高通量分析期间密集处理样品的广泛应用。
We describe a non-invasive technique for determining pH in biomolecular NMR sample using buffer components (formate, tris, piperazine, and imidazole) as internal pH indicators, whose H-1 NMR chemical shifts are sensitive to pH in a range from 2.5 to 9.8. This method is suitable for a wide range of applications where samples are handled intensively during NMR titrations or in high throughput analysis in structural genomics or metabolomics.