The effects of hyaluronic acid on articular chondrocytes
The effects of hyaluronic acid on articular chondrocytes
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DOI：
10.1302/0301-620x.87b8
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发表时间：
2005-08-01
影响因子：
--
通讯作者：
Bentley, G
中科院分区：
文献类型：
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作者：
Akmal, M;Singh, A;Bentley, G
本研究的目的是检查透明质酸补充对体外软骨细胞代谢的影响。关节内透明质酸注射的临床益处被认为是通过改善关节润滑而发生的。最近的研究表明，外源性透明质酸被纳入关节软骨中，它可能通过CD 44受体对软骨细胞产生直接的生物学效应。这些细胞在含有不同浓度透明质酸的培养基中培养。结果表明，透明质酸对体外培养的牛关节软骨细胞有剂量依赖性的作用。低浓度的透明质酸（0.1 mg/mL和1 mg/mL）显著增加DNA、硫酸化糖胺聚糖和羟脯氨酸的合成。免疫组织学证实细胞表型的维持与软骨素-6-硫酸盐和II型胶原蛋白的基质沉积增加。这些发现证实了透明质酸对软骨细胞代谢的刺激作用。
The purpose of this study was to examine the effects of hyaluronic acid supplementation on chondrocyte metabolism in vitro. The clinical benefits of intra-articular hyaluronic acid injections are thought to occur through improved joint lubrication. Recent findings have shown that exogenous hyaluronic acid is incorporated into articular cartilage where it may have a direct biological effect on chondrocytes through CD44 receptors.Bovine articular chondrocytes were isolated and seeded into alginate constructs. These were cultured in medium containing hyaluronic acid at varying concentrations. Samples were assayed for biochemical and histological changes.There was a dose-dependent response to the exposure of hyaluronic acid to bovine articular chondrocytes in vitro. Low concentrations of hyaluronic acid (0.1 mg/mL and 1 mg/mL) significantly increase DNA, sulphated glycosaminoglycan and hydroxyproline synthesis. Immunohistology confirmed the maintenance of cell phenotype with increased matrix deposition of chondroitin-6-sulphate and collagen type II. These findings confirm a stimulatory effect of hyaluronic acid on chondrocyte metabolism.