1,25(OH)2-vitamin D3 induces translocation of the vitamin D receptor (VDR) to the plasma membrane in skeletal muscle cells
1,25(OH)2-vitamin D3 induces translocation of the vitamin D receptor (VDR) to the plasma membrane in skeletal muscle cells
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DOI：
10.1002/jcb.10191
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发表时间：
2002-01-01
影响因子：
4
通讯作者：
Boland, RL
中科院分区：
文献类型：
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作者：
Capiati, D;Benassati, S;Boland, RL
1，25-二羟基维生素D-3（1，25（OH）（2）D-3），维生素D3的代谢活性形式，通过两种不同的机制发挥作用。除了通过特异性细胞内维生素D受体（VDR）调节基因表达外，1，25（OH 2）D-3还诱导快速的非转录反应，包括刺激跨膜信号转导途径。第二信使的激活支持了这样的假设，即膜结合类固醇受体类似于那些介导肽激素生物学存在。骨骼肌是1，25（OH）2D 3的靶组织。鸡胚胎骨骼肌细胞（成肌细胞/肌管）对维生素D有基因组和非基因组反应。本研究提供的证据表明，短期（1-10 min）1，25（OH）（2）D-3处理可诱导鸡成肌细胞VDR从核转位到微粒体。这种易位被秋水仙碱、染料木黄酮或除莠霉素阻断，表明微管转运和酪氨酸激酶参与受体的重新定位。通过质膜的分离，证明了该激素特别地增加该级分中的VIDR的量。这些结果表明，核VDR可能是介导1，25（OH）2D 3在鸡成肌细胞的非基因组效应的受体。(C)2002 Wiley-Liss，Inc.
1,25-dihydroxy-vitamin D-3 (1,25(OH)(2)D-3), the hormonally active form of vitamin D3, acts through two different mechanisms. In addition to regulating gene expression via the specific intracellular vitamin D receptor (VDR), 1,25(OH2)D-3 induces rapid, non-transcriptional responses involving stimulation of transmembrane signal transduction pathways. The activation of second messengers supports the hypothesis that a membrane-bound steroid receptor similar to those that mediate peptide hormone biology exists. Skeletal muscle is a target tissue for 1,25(OH)2D3. Avian embryonic skeletal muscle cells (myoblasts/myotubes) have been shown to respond both genomically and non-genomically to the hormone, The present study provides evidence indicating that short-term treatment (1-10 min) with 1,25(OH)(2)D-3 induces translocation of the VDR from the nuclear to the microsomal fraction in chick myoblasts. This translocation is blocked by colchicine, genistein, or herbimycin, suggesting the involvement of microtubular transport and tyrosine kinase/s in the relocation of the receptor. By isolation of plasma membranes, it was demonstrated that the hormone increases the amounts of VIDR specifically in this fraction. These results suggest that the nuclear VDR may be the receptor that mediates the non-genomic effects of 1,25(OH)2D3 in chick myoblasts. (C) 2002 Wiley-Liss, Inc.