COMPARISON OF PROTEOLYTIC CLEAVAGE PATTERNS OF ALPHA-TUBULINS AND BETA-TUBULINS FROM TAXONOMICALLY DISTANT SPECIES
COMPARISON OF PROTEOLYTIC CLEAVAGE PATTERNS OF ALPHA-TUBULINS AND BETA-TUBULINS FROM TAXONOMICALLY DISTANT SPECIES
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DOI：
10.1016/0022-2836(81)90262-x
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发表时间：
1981-01-01
影响因子：
5.6
通讯作者：
FARRELL, K
中科院分区：
文献类型：
--
作者：
LITTLE, M;LUDUENA, RF;FARRELL, K
.alpha。在十二烷基硫酸钠存在下，通过聚丙烯酰胺凝胶电泳分离来自几个分类学上较远物种的微管蛋白的β-链和β-链，并通过避免染色和酸性条件的程序进行分离。所有β-链具有相同的电泳迁移率，但一些低等真核生物的α-链比其他真核生物迁移得更快。用金黄色葡萄球菌蛋白酶对亚基进行有限的蛋白水解消化，然后在15％丙烯酰胺凝胶上进行电泳，导致所有β-链产生非常相似的肽模式，但α-链产生多种模式。来自海胆卵和精子鞭毛外双联体的α-微管蛋白的肽模式明显不同，而牛脑和肾的肽模式是相同的。牛和角鲨脑的α-微管蛋白肽模式也相同，而乌贼脑的肽模式则截然不同。在来自线虫 Urechis caupo 和海胆 Lytechinus pictus 和 Strongylocentrotus purpuratus 的精子鞭毛微管蛋白的 α-链肽模式之间发现了很强的相似性，这表明来自非常不同物种的功能相似的微管蛋白可能比来自同一生物体的功能不同的微管蛋白更密切相关。来自蕨类蕨类植物的精子鞭毛和单细胞双鞭毛藻莱茵衣藻的鞭毛的微管蛋白的独特且非常相似的微管蛋白α链肽模式提供了植物精子鞭毛从原生生物鞭毛进化的证据。
The .alpha. and .beta.-chains of tubulins from several taxonomically distant species were separated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and isolated by a procedure that avoided stains and acidic conditions. All the .beta.-chains had the same electrophoretic mobility, but the .alpha.-chains of some of the lower eukaryotes migrated faster than the rest. Limited proteolytic digestion of the subunits with Staphylococcus aureus protease, followed by electrophoresis on 15% acrylamide gels, resulted in very similar peptide patterns for all the .beta.-chains, but a variety of patterns for the .alpha.-chains. The peptide patterns of .alpha.-tubulins from sea urchin egg and sperm flagellar outer doublets were markedly different, whereas those of bovine brain and kidney were identical. Bovine and dogfish brain .alpha.-tubulin peptide patterns were also identical, in contrast to the very different one of squid brain. Strong similarities were found between the .alpha.-chain peptide patterns of sperm flagellar tubulin from the echiurid worm Urechis caupo and the sea urchins Lytechinus pictus and Strongylocentrotus purpuratus, indicating that functionally similar tubulins from very different species can be more closely related than functionally different tubulins from the same organism. Evidence for the evolution of plant sperm flagella from protistan flagella was provided by the distinctive and very similar .alpha.-chain peptide patterns of tubulin from sperm flagella of the bracken fern Pteridium aquilinum and the flagella of the unicellular biflagellate alga Chlamydomonas reinhardtii.