Correlation of serpin-protease expression by comparative analysis of real-time PCR profiling data
Correlation of serpin-protease expression by comparative analysis of real-time PCR profiling data
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DOI：
10.1016/j.ygeno.2006.03.017
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发表时间：
2006-08-01
期刊：
影响因子：
4.4
通讯作者：
Tsu, Christopher A.
中科院分区：
文献类型：
--
作者：
Badola, Sunita;Spurling, Heidi;Tsu, Christopher A.
蛋白酶活性不平衡在癌症和炎症等疾病的进展中早已被认识到。蛇肽是最大的内源性蛋白酶抑制剂家族，针对多种丝氨酸和半胱氨酸蛋白酶，并在许多生理和病理状态中发挥作用。20种蛇蛋白和105种丝氨酸和半胱氨酸蛋白酶的表达谱在正常和患病的人体组织中被测定。一般来说，serpins在正常和病变组织中的表达都受到高度限制，这表明这些蛋白酶抑制剂具有明确的生理作用。健康组织中特定蛇形蛋白蛋白酶对表达的高相关性通常预示着生物相互作用。最引人注目的发现是在病变组织中蛋白酶及其同源抑制剂之间的表达调节中观察到的戏剧性变化。失去受调节的蛇形蛋白蛋白酶匹配表达可能是病理状态下观察到的蛋白酶活性不平衡的基础。(c) 2006爱思唯尔公司版权所有。
Imbalanced protease activity has long been recognized in the progression of disease states such as cancer and inflammation. Serpins, the largest family of endogenous protease inhibitors, target a wide variety of serine and cysteine proteases and play a role in a number of physiological and pathological states. The expression profiles of 20 serpins and 105 serine and cysteine proteases were determined across a panel of normal and diseased human tissues. In general, expression of serpins was, highly restricted in both normal and diseased tissues, suggesting defined physiological roles for these protease inhibitors. A high correlation in expression for a particular serpin-protease pair in healthy tissues was often predictive of a biological interaction. The most striking finding was the dramatic change observed in the regulation of expression between proteases and their cognate inhibitors in diseased tissues. The loss of regulated serpin-protease matched expression may underlie the imbalanced protease activity observed in pathological states. (c) 2006 Elsevier Inc. All rights reserved.