The bacterial enhancer-binding protein NtrC as a molecular machine

The bacterial enhancer-binding protein NtrC as a molecular machine
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DOI:
10.1101/sqb.1998.63.157
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发表时间:
1998-01-01
期刊:
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子:
--
通讯作者:
Kustu, S
Kustu, S
中科院分区:
其他
文献类型:
--
作者:
Rombel, I;North, A;Kustu, S

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方法蛋白质的制备、纯化及活性测定。如前所述(Porter等1993; Flashner等1995; North等1996; North和Kustu 1997; I. Rombel等人,未公开的)。除非另有说明,蛋白质浓度是指二聚体。的能力
METHODSOverproduction and purification of proteins and assays of their activities. Proteins were overexpressed and purified as described previously (Porter et al. 1993; Flashner et al. 1995; North et al. 1996; North and Kustu 1997; I. Rombel et al., unpubl.). Unless noted otherwise, protein concentrations refer to dimers. The ability of