Delayed rehabilitation lessens brain injury and improves recovery after intracerebral hemorrhage in rats
Delayed rehabilitation lessens brain injury and improves recovery after intracerebral hemorrhage in rats
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DOI：
10.1016/j.brainres.2008.11.038
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发表时间：
2009-01-28
期刊：
影响因子：
2.9
通讯作者：
Colbourne, Frederick
中科院分区：
文献类型：
--
作者：
Auriat, Angela M.;Colbourne, Frederick
康复治疗可促进大鼠脑出血后的康复。在某些情况下，大脑损伤会减轻。在这项研究中，我们测试了环境丰富(EE)与熟练的REACH训练相结合是否能促进大鼠脑出血后的恢复并减轻脑损伤。通过立体定向注射胶原酶，形成一个中等大小的纹状体脑出血。脑出血后1周，将大鼠分为康复组(康复组)和对照组(CONT)。康复大鼠每天接受15小时的EE训练和4次15分钟的REACH训练，每周5天，为期2周。CONT大鼠留在标准组笼中。在脑出血后4周和6周分别评定熟练的伸手(楼梯试验)、行走(水平梯子)和前肢使用偏斜(柱状试验)。于脑出血后46d计算病灶体积、胼胝体体积和皮质厚度。康复治疗使损伤体积减少了28%(p=0.019)，而不影响胼胝体体积(p=0.405)或皮质厚度(p=0.300)，从而表明保护作用是由于减轻了纹状体损伤。此外，康复显著提高了脑出血后4周(p=0.002)和6周(p<；0.001)楼梯器械的熟练伸展能力。阶梯试验在4周时获得一过性益处(p=0.021)。出乎意料的是，康复治疗在4周(p=0.045)和6周(p=0.041)减少了对侧至脑出血肢体的自发使用。总而言之，EE和REACH训练的结合显著减少了损伤体积(纹状体损伤)，同时提高了熟练的伸展和行走能力。这些发现鼓励基底节出血患者使用早期康复治疗。(C)2008爱思唯尔B.V.保留所有权利。
Rehabilitation improves recovery after intracerebral hemorrhage (ICH) in rats. In some cases, brain damage is attenuated. In this study, we tested whether environmental enrichment (EE) combined with skilled reach training improves recovery and lessens brain injury after ICH in rats. Collagenase was injected stereotaxically to produce a moderate-sized striatal ICH. One week after ICH rats were either placed into a rehabilitation (REHAB) or control (CONT) condition. The REHAB rats received 15 h of EE and four 15-minute reach-training sessions daily over 5 days a week for 2 weeks. The CONT rats stayed in standard group cages. Skilled reaching (staircase test), walking (horizontal ladder) and forelimb use bias (cylinder test) were assessed at 4 and 6 weeks after ICH. Lesion volume, corpus callosum volume and cortical thickness were calculated 46 days after ICH. The REHAB treatment reduced lesion volume by 28% (p=0.019) without affecting the corpus callosum volume (p=0.405) or cortical thickness (p=0.300), thus indicating that protection was due to lessening striatal injury. As well, REHAB significantly improved skilled reaching ability in the staircase apparatus at 4 (p=0.002) and 6 weeks (p < 0.001) post-ICH. Transient benefit was obtained in the ladder test at 4 weeks (p=0.021). Unexpectedly, REHAB treatment lessened spontaneous use of the contralateral-to-ICH limb at 4 (p=0.045) and 6 weeks (p=0.041). In summary, the combination of EE and reach training significantly attenuates lesion volume (striatal injury) while improving skilled reaching and walking ability. These findings encourage the use of early rehabilitation therapies in patients suffering from basal ganglia hemorrhaging. (C) 2008 Elsevier B.V. All rights reserved.