Radiation-induced endothelial cell loss and reduction of the relative magnitude of the blood flow in the rat spinal cord
Radiation-induced endothelial cell loss and reduction of the relative magnitude of the blood flow in the rat spinal cord
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DOI：
10.1016/j.brainres.2014.06.014
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发表时间：
2014-10
期刊：
影响因子：
2.9
通讯作者：
Jing Zhang;Li Wei;Wan-Liang Sun;Lei Wang;Wei-Jing Zhang;Hua You
中科院分区：
文献类型：
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作者：
Jing Zhang;Li Wei;Wan-Liang Sun;Lei Wang;Wei-Jing Zhang;Hua You
本研究的主要目的是检查内皮细胞密度的时间依赖性变化，发生在第一个180天后照射的脊髓和这些变化在脊髓血流的功能作用。用60 Co照射器照射大鼠颈脊髓（C2-T2节段），剂量为30戈伊。照射后180天观察到前肢运动功能的显著丧失。脊髓前角神经元数目在照射后3d开始明显减少，至照射后90d最少。在白色物质中，从照射后14天开始观察到内皮细胞密度的显著降低，在灰质中，从照射后3天开始观察到内皮细胞密度的显著降低。白色物质在照射后30天和灰质在照射后60天达到最低内皮细胞密度。从照射后3天起，在白色物质和灰质中观察到微血管密度的显著降低。在白色质和灰质中，微血管密度在照射后90天达到最低。从照射后21天开始观察到脊髓血流量的相对大小显著减少。脊髓血流量的相对幅度在照射后90天达到最低。我们没有发现任何脱髓鞘的迹象。结果显示，单次戈伊照射可导致前肢运动功能受损，神经元数量减少，内皮细胞密度、微血管密度和脊髓血流量相对幅度降低。然而，30-戈伊单次剂量照射不足以诱导大鼠脊髓脱髓鞘。
The main purpose of the present study was to examine the time-dependent alterations in the endothelial cell density that occur in the first 180 days after irradiation of the spinal cord and the functional role of these alterations in the spinal cord blood flow. An irradiated cervical spinal cord rat model (C2-T2 segment) was generated using a60Co irradiator to deliver 30 Gy. A significant loss of forelimb motor function was observed 180 days post-irradiation. The number of neurons in the anterior horn of the spinal cord began to decrease significantly 3 days post-irradiation compared with normal controls, reaching the lowest number at 90 days post-irradiation. A significant reduction in the endothelial cell density was observed from 14 days post-irradiation in the white matter and from 3 days post-irradiation in the gray matter. The lowest endothelial cell density was reached at 30 days post-irradiation in the white matter and at 60 days post-irradiation in the gray matter. A significant reduction in the microvessel density was observed from 3 days post-irradiation in both the white matter and the gray matter. The lowest microvessel density was reached at 90 days post-irradiation in both the white matter and the gray matter. A significant reduction in the relative magnitude of spinal cord blood flow was observed from 21 days post-irradiation. The lowest relative magnitude of spinal cord blood flow was reached at 90 days post-irradiation. We did not find any evidence of demyelination. The results revealed that a single 30-Gy irradiation dose resulted in impaired forelimb motor function, a decreased number of neurons, and reduced endothelial cell density, microvessel density and relative magnitude of spinal cord blood flow. However, a 30-Gy single-dose irradiation was not sufficient to induce demyelination in the rat spinal cord.