Interaction of colloidal gold-labelled glucosylated albumin with endothelial cell monolayers: comparison between cryofixation and glutaraldehyde fixation.
Interaction of colloidal gold-labelled glucosylated albumin with endothelial cell monolayers: comparison between cryofixation and glutaraldehyde fixation.
复制标题
胶体金标记的糖基化白蛋白与内皮细胞单层的相互作用：冷冻固定和戊二醛固定之间的比较。
DOI：
10.1002/jemt.1070300306
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Williams,SK
中科院分区：
文献类型：
--
作者：
Hoying,JB;Chen,SC;Williams,SK
Bovine aortic endothelial cells (BAEC) were exposed to glucosylated albumin‐gold complexes (GgA), and the distribution of the tracers was compared after cryofixation and after glutaraldehyde fixation. Morphometric analysis revealed differences in the GgA distribution depending upon the method of fixation used. In BAEC monolayers cryofixed after 3 min of incubation with GgA, tracer was observed in predominately apically located vesicular elements. After 16 min of incubation, all vesicular elements were labelled, and multivesicular bodies were the prominent labelled structure. In contrast, chemically fixed monolayers exhibited a heterogeneous distribution of GgA within vesicular profiles after 3 min and 16 min of GgA incubation. The differences in tracer distribution depending upon the fixation method must be resolved before the mechanism of vesiclemediated endothelial cell transport function is defined and universally accepted. © 1995 Wiley‐Liss, Inc.
