Multi-scale mapping along the auditory hierarchy using high-resolution functional UltraSound in the awake ferret.
Multi-scale mapping along the auditory hierarchy using high-resolution functional UltraSound in the awake ferret.
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DOI：
10.7554/elife.35028
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发表时间：
2018-06-28
期刊：
影响因子：
7.7
通讯作者：
Boubenec Y
中科院分区：
文献类型：
--
作者：
Bimbard C;Demene C;Girard C;Radtke-Schuller S;Shamma S;Tanter M;Boubenec Y
神经科学中的一个主要挑战是在同一动物中跨多个空间尺度纵向监测整个大脑活动。功能性超声（fUS）是一种新兴技术，可提供大脑大部分脑血容量的图像。在这里，我们第一次展示了它的能力，以解决在清醒的动物，无论是在小结构内精确映射和区分感官反应的感觉系统在多个尺度上的功能组织，并阐明自上而下的投影的连接方案的结构之间。我们证明，fUS在清醒的雪貂的听觉通路中提供了稳定（数天），但快速，高分辨率的3D音调定位图，从而揭示了其前所未有的功能分辨率（100/300µm）。这是在四个不同的大脑区域进行的，包括非常小的（1-2 mm 3大小），位于皮层下（8 mm深）和以前未描述的雪貂结构。此外，我们使用fUS来映射从额叶皮质（感觉反应调制的关键来源）到听觉皮质的长距离投射。
A major challenge in neuroscience is to longitudinally monitor whole brain activity across multiple spatial scales in the same animal. Functional UltraSound (fUS) is an emerging technology that offers images of cerebral blood volume over large brain portions. Here we show for the first time its capability to resolve the functional organization of sensory systems at multiple scales in awake animals, both within small structures by precisely mapping and differentiating sensory responses, and between structures by elucidating the connectivity scheme of top-down projections. We demonstrate that fUS provides stable (over days), yet rapid, highly-resolved 3D tonotopic maps in the auditory pathway of awake ferrets, thus revealing its unprecedented functional resolution (100/300µm). This was performed in four different brain regions, including very small (1–2 mm3 size), deeply situated subcortical (8 mm deep) and previously undescribed structures in the ferret. Furthermore, we used fUS to map long-distance projections from frontal cortex, a key source of sensory response modulation, to auditory cortex.