Strain-dependent induction of cytokine profiles in the gut by orally administered Lactobacillus strains
Strain-dependent induction of cytokine profiles in the gut by orally administered Lactobacillus strains
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DOI：
10.1016/s0264-410x(99)00378-3
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发表时间：
2000-05-22
期刊：
影响因子：
5.5
通讯作者：
Claassen, E
中科院分区：
文献类型：
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作者：
Maassen, CBM;van Holten-Neelen, C;Claassen, E
不同的乳杆菌菌株经常用于消费食品。此外，含有新型表达载体的重组乳酸菌现在可用于免疫治疗应用，如口服疫苗接种策略和自身免疫性疾病的T细胞耐受性诱导方法。无论是食品还是临床应用，正确选择野生型菌株都是至关重要的。为此，研究人员分析了8种不同的常见乳酸菌菌株对促炎性和抗炎性细胞因子、肠道中产生iga的浆细胞的粘膜诱导，以及对一种非肠外给药抗原的全身抗体反应。对肠道绒毛细胞因子产生细胞的免疫组化分析显示，口服野生型乳杆菌菌株对细胞因子IL-1 α、ifn - γ、IL-4和IL-10没有显著的诱导作用，相反，口服乳杆菌、罗伊氏乳杆菌和短乳杆菌可诱导促炎/Th1细胞因子tnf - α、IL-2和/或IL-1 β的表达。口服这两株菌株和L，发酵菌也能显著增强IgG对肠外给药鸡γ球蛋白（TNP-CGG）的免疫应答，而其他5株菌株则没有这种增效作用。与鼠乳杆菌相比，罗伊氏乳杆菌诱导的IgG2a水平相对较高，而鼠乳杆菌是一种不调节的乳杆菌菌株。这些发现表明，不同的乳杆菌菌株诱导不同的粘膜细胞因子谱和具有不同的内在佐剂性。这表明合理的乳酸菌菌株选择提供了一种影响细胞因子表达从而影响免疫应答的策略。(C) 2000 Elsevier Science Ltd.版权所有。
Different Lactobacillus strains are frequently used in consumer food products. In addition, recombinant lactobacilli which contain novel expression vectors can now be used in immunotherapeutic applications such as oral vaccination strategies and in T cell tolerance induction approaches for autoimmune disease. Both for food and clinical applications of lactobacilli, proper selection of wild type strains is crucial.For that purpose, eight different common Lactobacillus strains were analysed with respect to mucosal induction of pro- and anti-inflammatory cytokines, IgA-producing plasma cells in the gut, as well as systemic antibody responses against a parenterally administered antigen. Immunohistochemical analysis of cytokine-producing cells in the gut villi showed no significant induction of the cytokines IL-1 alpha, IFN-gamma, IL-4 or IL-10 after oral administration of wild type Lactobacillus strains, In contrast, oral administration of L, reuteri and L. brevis induced expression of the proinflammatory/Th1 cytokines TNF-alpha, IL-2 and/or IL-1 beta. Oral administration of these two strains and L, fermentum also significantly enhanced the IgG response against parenterally administered haptenated chicken gamma globulin (TNP-CGG), The five other strains did not show this adjuvanticity. L. reuteri induced relatively high levels of IgG2a compared to L. murines, a nonadjuving Lactobacillus strain.These findings imply that different Lactobacillus strains induce distinct mucosal cytokine profiles and possess differential intrinsic adjuvanticity. This suggests that rational Lactobacillus strain selection provides a strategy to influence cytokine expression and thereby influence immune responses. (C) 2000 Elsevier Science Ltd. All rights reserved.