The molecular karyotype of Leishmania major and mapping of α and β tubulin gene families to multiple unlinked chromosomal loci
The molecular karyotype of Leishmania major and mapping of α and β tubulin gene families to multiple unlinked chromosomal loci
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大型利什曼原虫的分子核型以及 α 和 β 微管蛋白基因家族到多个非连锁染色体位点的定位
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
N. Samaras
中科院分区：
文献类型：
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作者：
T. Spithill;N. Samaras
通过基因组Southern印迹分析和脉冲梯度凝胶(PFG)凝胶电泳法将微管蛋白基因定位到染色体上，研究了大利什曼原虫基因组中微管蛋白基因的排列。α-微管蛋白基因以2.4kb的PstI片段串联阵列形式存在。β-微管蛋白基因是由3.9kb的AvAI或PvuI片段组成的串联阵列，但在其他基因组DNA片段上也发现了额外的基因。利用PFG凝胶电泳法，从大斑灵芝的前鞭毛体中释放出染色体大小的DNA分子，分成至少17条染色体条带，大小约为400-4000 kb。一些带可能以非等摩尔的数量存在，这表明可能有17条以上的染色体。所有的α-微管蛋白基因都定位在一条带上(7号染色体)。β-微管蛋白基因定位于4条带(第6、10、16和17号染色体)。这表明，阿尔法和β-微管蛋白基因家族在主要乳杆菌中是不连锁的。α-微管蛋白串联阵列只有一个染色体位点，而β-微管蛋白基因既以串联阵列的形式存在，又以分散基因的形式存在于四个染色体位点。
The arrangement of tubulin genes in the genome of the protozoan parasite Leishmania major was studied by genomic Southern blot analysis and mapping of genes to chromosomes fractionated by pulsed field gradient gel (PFG) electrophoresis. alpha-tubulin genes exist as a tandem array of 2.4 kb PstI fragments. beta-tubulin genes are found as a tandem array of 3.9 kb AvaI or PvuI fragments, but additional genes are also found on other genomic DNA fragments. Chromosome-sized DNA molecules released from promastigotes of L. major were fractionated into at least 17 chromosome bands of approximate size 400-4000 kb by PFG gel electrophoresis. Some bands may be present in non-equimolar amounts suggesting that there may be more than 17 chromosomes. All alpha-tubulin genes were localized to a single band (chromosome 7). beta-tubulin genes were localized to four bands (chromosomes 6, 10, 16 and 17). This shows that the alpha- and beta- tubulin gene families are unlinked in L. major. There is a single chromosomal locus for the alpha-tubulin tandem array whereas beta-tubulin genes exist both as a tandem array and as dispersed genes at four chromosomal loci.