THE ROLE OF MACROPHAGES IN THE UTERINE RESPONSE TO PREGNANCY
THE ROLE OF MACROPHAGES IN THE UTERINE RESPONSE TO PREGNANCY
复制标题
DOI：
10.1016/s0143-4004(05)80192-4
复制
发表时间：
1990-11-01
期刊：
影响因子：
3.8
通讯作者：
HUNT, JS
中科院分区：
文献类型：
--
作者：
HUNT, JS
免疫组织学实验已经建立了巨噬细胞在妊娠子宫中的分布模式，并积累了一些关于这些细胞潜在的化学引诱物的数据。几种研究方法的结果表明，与其他组织中的巨噬细胞一样，这些细胞是多功能的。进一步的实验可能在技术上要求很高，因为有迹象表明，复杂的前列腺素-细胞因子网络调节子宫巨噬细胞的活动。子宫巨噬细胞合成细胞因子的问题特别有趣（Hunt，1989 a，Journal of Reproductive Immunology，116，1-17），并且特别困难。这些形态上异质的细胞散布在整个子宫中，与合成某些相同分子的其他类型的细胞一起，分离所需的操作很容易影响transnasient基因的转录（Taniguchi，1988）。因此，许多实验必须使用免疫组织学和原位杂交在完整组织上进行。虽然这些非凡的细胞无疑有助于怀孕所需的发育事件，但子宫巨噬细胞可能具有有害和有益的影响，特别是在感染的情况下。干扰素和细菌脂多糖（LPS）的激活破坏了正常的合成模式，并导致生物活性蛋白质和脂质浓度增加的分泌。更高水平的IL-1（Romero等，1989）、TNF-α、TNF-α、TNF-α、TNF-α和TNF-α。（凯西等，1989）和IL-6（Romero等，1990），以及增加的胡萝卜素水平（Romero等，1987），所有活化的巨噬细胞的前产物，与由于感染导致的妊娠终止有关。这些分子中的一些可能会导致早产，而另一些可能会改变细胞功能。我们实验室最近的实验表明，尽管成孔细胞携带LPS受体，但在革兰氏阴性细菌感染期间对这些细胞的损伤更可能是由于接触巨噬细胞衍生的分子而不是暴露于LPS（Hunt等，1989）。在这一领域的进一步试验可能会导致治疗方案，将是有效的，以减轻这一重大的健康问题。
Immunohistological experiments have estiablished patterns of distribution of macrophages in the pregnant uterus and some data have been accumulated on potential chemoattractants for these cells. The results of several lines of inquiry indicate that, as with macrophages in other tissues, these cells are multifunctional. Further experimentation is likely to be technically demanding because of indications that intricate hormone-prostaglandin-cytokine networks regulate uterine macrophage activities. The question of cytokine synthesis by uterine macrophages is particularly intriguing (Hunt, 1989a, Journal of Reproductive Immunology, 116, 1-17) and particularly difficult. These morphologically heterogenous cells are interspersed throughout the uterus with other types of cells that syntehsize some of the same molecules, and the manipulation required for isolation could easily affect trnasient gene transcription (Taniguchi, 1988). Thus, many experiments must be performed on intact tissues using immunohistology and in situ hybridization. Although the remarkable cells undoubtedly contribute to the required developmental events of pregnancy, uterine macrophages may have detrimental as well as beneficial effects, particularly in cases of infection. Activation by interferons and bacterial lipopolysaccharides (LPS) disrupts normal synthetic patterns, and results in secretion of increased concentrations of bioactive proteins and lipids. Higher levels of IL-1 (Romero et al, 1989), TNF-.alpha. (Casey et al, 1989) and IL-6 (Romero et al, 1990), as well as increased levels of prostaglandins (Romero et al, 1987), all preoducts of activated macrophages, are associated with pregnancy termination due to infection. Some of these molecules could induce premature labour, and others might alter cellular functions. Recent experiments in our laboratory suggest although torphoblast cells bear LPS receptors, damage to these cells during gram negative bacterial infections is more likely to result from encounters with macrophage-derived molecules than form exposure to LPS (Hunt et al, 1989). Further experimentation in this area might lead to treatment regimens that would be effective in alleviating this major health problem.