Murine cytomegalovirus induces a polyfunctional CD4 T cell response
Murine cytomegalovirus induces a polyfunctional CD4 T cell response
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DOI：
10.4049/jimmunol.180.10.6472
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发表时间：
2008-05-15
影响因子：
4.4
通讯作者：
Benedict, Chris A.
中科院分区：
文献类型：
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作者：
Arens, Ramon;Wang, Peng;Benedict, Chris A.
CD4T淋巴细胞通过向CD8T细胞和B细胞提供帮助以及通过直接的抗病毒活性来调节对大多数病毒的适应性免疫反应。目前，尚无小鼠巨细胞病毒(MCMV)特异性的CD4T细胞应答。在这项研究中，我们鉴定和表征了15个针对MCMV表位的I-A(B)限制性CD4T细胞反应。在急性感染期间，CD4T细胞在脾和肺中积聚到高水平，并产生多种细胞因子(干扰素-γ、肿瘤坏死因子、IL-2、IL-10和IL-17)。有趣的是，在抗原表位特异的细胞中，IL-17和干扰素-γ的产生被发现是相互排斥的。识别来自m09的多肽的CD4T细胞只有在感染后才能检测到，并显示出独特的细胞因子产生谱。总之，这项研究揭示了MCMV特异性的CD4T细胞反应是复杂的和功能多样化的，突出了它在控制这种持久性病原体方面的重要作用。
CD4 T lymphocytes regulate the adaptive immune response to most viruses, both by providing help to CD8 T cells and B cells as well as through direct antiviral activity. Currently, no mouse cytomegalovirus (MCMV)-specific CD4 T cell responses are known. In this study, we identify and characterize 15 I-A(b)-restricted CD4 T cell responses specific for MCMV epitopes. CD4 T cells accumulate to high levels in the spleen and lungs during acute infection and produce multiple cytokines (IFN-gamma, TNF, IL-2, IL-10, and IL-17). Interestingly, IL-17 and IFN-gamma production within epitope-specific cells was found to he mutually exclusive. CD4 T cells recognizing a peptide derived from m09 were only detectable at later times of infection and displayed a unique cytokine production profile. In total, this study reveals that the MCMV-specific CD4 T cell response is complex and functionally diverse, highlighting its important role in controlling this persistent pathogen.