Single-Cell Analysis of Circulating Tumor Cells as a Window into Tumor Heterogeneity.
Single-Cell Analysis of Circulating Tumor Cells as a Window into Tumor Heterogeneity.
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DOI：
10.1101/sqb.2016.81.031120
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Haber DA
中科院分区：
文献类型：
--
作者：
Miyamoto DT;Ting DT;Toner M;Maheswaran S;Haber DA
微流控方法的最新进展使得能够高效分离癌症患者外周血中的循环肿瘤细胞（CTCs），并对其进行详细的分子特征分析。对CTCs的单细胞分子分析揭示了CTC群体中细胞内存在极大程度的异质性，这反映了不同患者之间的异质性以及每个患者体内肿瘤潜在的异质性。这些研究使得能够识别在难治性肿瘤中可能共存的异质性耐药机制。CTC分析还能够对患者进行连续的无创监测，并且能够捕捉随着时间推移肿瘤异质性的出现，无论是由于基因不稳定导致的肿瘤演变还是细胞可塑性。通过对CTCs的研究揭示的肿瘤内异质性的存在及其程度，对于理解和预测多种实体瘤治疗耐药性的发展以及制定有效的癌症治疗策略具有重要的临床意义。
Recent advances in microfluidic approaches have enabled the efficient isolation and detailed molecular characterization of circulating tumor cells (CTCs) in the peripheral blood of patients with cancer. Single-cell molecular analyses of CTCs reveal a tremendous degree of intracellular heterogeneity in CTC populations, reflective of heterogeneity across different patients as well as the underlying heterogeneity of tumors within each individual patient. These studies have enabled the identification of heterogeneous drug resistance mechanisms that can coexist in treatment refractory tumors. CTC analyses also enable serial noninvasive monitoring in patients and can capture the emergence of tumor heterogeneity over time, whether due to tumor evolution through genetic instability or through cellular plasticity. The presence and extent of intratumoral heterogeneity as revealed through the study of CTCs have important clinical implications for understanding and predicting the development of treatment resistance in a variety of solid tumors and for formulating appropriate therapeutic strategies in the effective treatment of cancer.
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影响因子：
14.8
作者：
通讯作者：
--
DOI：
10.1158/1078-0432.ccr-15-0137
发表时间：
2016-03-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Beltran H;Jendrisak A;Landers M;Mosquera JM;Kossai M;Louw J;Krupa R;Graf RP;Schreiber NA;Nanus DM;Tagawa ST;Marrinucci D;Dittamore R;Scher HI
通讯作者：
Scher HI
影响因子：
8.4
作者：
FIDLER, IJ
通讯作者：
FIDLER, IJ
DOI：
10.1056/nejmoa1315815
发表时间：
2014-09-11
期刊：
The New England journal of medicine
影响因子：
--
作者：
Antonarakis ES;Lu C;Wang H;Luber B;Nakazawa M;Roeser JC;Chen Y;Mohammad TA;Chen Y;Fedor HL;Lotan TL;Zheng Q;De Marzo AM;Isaacs JT;Isaacs WB;Nadal R;Paller CJ;Denmeade SR;Carducci MA;Eisenberger MA;Luo J
通讯作者：
Luo J
影响因子：
64.8
作者：
Jordan, Nicole Vincent;Bardia, Aditya;Wittner, Ben S.;Benes, Cyril;Ligorio, Matteo;Zheng, Yu;Yu, Min;Sundaresan, Tilak K.;Licausi, Joseph A.;Desai, Rushil;O'Keefe, Ryan M.;Ebright, Richard Y.;Boukhali, Myriam;Sil, Srinjoy;Onozato, Maristela L.;Iafrate, Anthony J.;Kapur, Ravi;Sgroi, Dennis;Ting, David T.;Toner, Mehmet;Ramaswamy, Sridhar;Haas, Wilhelm;Maheswaran, Shyamala;Haber, Daniel A.
通讯作者：
Haber, Daniel A.