Decreased keratinocyte Proline‐Rich protein expression in cutaneous T‐cell lymphoma
Decreased keratinocyte Proline‐Rich protein expression in cutaneous T‐cell lymphoma
复制标题
皮肤 T 细胞淋巴瘤中角质形成细胞富含脯氨酸蛋白表达减少
DOI：
10.1002/cia2.12240
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发表时间：
2022
影响因子：
1
通讯作者：
Sato Shinichi
中科院分区：
文献类型：
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作者：
Hisamoto Teruyoshi;Suga Hiraku;Omori Issey;Mizuno Yuka;Oka Kenta;Boki Hikari;Takahashi‐Shishido Naomi;Oka Tomonori;Miyagaki Tomomitsu;Sugaya Makoto;Sato Shinichi
皮肤T细胞淋巴瘤（CTCLs）通常伴有皮肤干燥和皮肤屏障功能障碍，这也见于特应性皮炎（AD）。角化细胞脯氨酸富蛋白（KPRP）在AD表皮中的表达减少，导致屏障缺陷和进一步的过敏性炎症。目的分析KPRP在CTCL中的表达。材料与方法收集CTCL和健康对照组皮肤样本，分析皮肤组织中KPRP mRNA的表达情况。我们还对KPRP蛋白进行了免疫组化。结果与正常皮肤相比，角质细胞富含脯氨酸蛋白（KPRP） mRNA表达降低。KPRP的表达与聚丝蛋白和loricrin呈正相关，与血清白细胞介素- 2受体水平呈负相关。免疫组织化学染色显示，正常皮肤和早期CTCL的表皮颗粒层上部检测到KPRP，但在晚期CTCL中很少检测到KPRP。结论CTCL中KPRP表达明显降低。
BackgroundCutaneous T‐cell lymphomas (CTCLs) often have skin dryness and skin barrier dysfunctions, which are also seen in atopic dermatitis (AD). Expression of keratinocyte proline‐rich protein (KPRP) was decreased in the epidermis of AD, which led to barrier defects and further allergic inflammation.ObjectivesThe aim of this study was to analyze KPRP expression in CTCL.Materials & MethodsSkin samples of CTCL and healthy control were collected, and KPRP mRNA expression in the skin tissue was analyzed. We also performed immunohistochemistry of KPRP protein.ResultsKeratinocyte proline‐rich protein (KPRP) mRNA expression was decreased compared with normal skin. KPRP expression was positively correlated with that of filaggrin and loricrin, and it was negatively correlated with serum interleukin‐2 receptor levels. Immunohistochemistry staining showed that KPRP was detected in upper part of granular layer of epidermis in normal skin and in early‐stage CTCL, but rarely detected in the advanced‐stage CTCL.ConclusionIn conclusion, we revealed the decrease in KPRP expression in CTCL.