Isolation and characterization of Whitewater Arroyo virus, a novel North American arenavirus
Isolation and characterization of Whitewater Arroyo virus, a novel North American arenavirus
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DOI：
10.1006/viro.1996.0512
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发表时间：
1996-10-01
期刊：
影响因子：
3.7
通讯作者：
Peters, CJ
中科院分区：
文献类型：
--
作者：
Fulhorst, CF;Bowen, MD;Peters, CJ
啮齿动物是每种特征明确的沙粒病毒的主要宿主。在本研究之前，塔迈阿密（TAM）病毒是唯一已知的北美本土沙粒病毒;它仅从佛罗里达南部分离出来，其主要宿主是棉鼠Sigmodon hispidus。最近，在从美国西南部采集的白腹瘤蛛中发现了沙粒病毒抗体。本研究的目的是分离和鉴定与N. albigula。从两株沙粒病毒抗体阳性的N.从新墨西哥州麦金利县的白水阿罗约收集的白姑鱼。双向血清学试验表明，WWA病毒的抗原性不同于其他沙粒病毒，但与TAM病毒的关系最为密切。核衣壳蛋白基因序列的系统发育分析表明，WWA病毒是一种新的沙粒病毒，与TAM病毒在遗传学上关系最密切。albigula表明WWA病毒在该物种中的感染可以是慢性的，因此N. albigula是该病毒的储存宿主。(C)出版社：Academic Press，Inc.
Rodents are principal hosts for each of the well-characterized arenaviruses. Prior to the present study, Tamiami (TAM) virus was the sole arenavirus known to be indigenous to North America; it has been isolated only from southern Florida where its primary host is the cotton rat Sigmodon hispidus. Recently, arenavirus antibody was found in Neotoma albigula woodrats collected from the southwestern United States. The purpose of the present study was to isolate and characterize the arenavirus associated with N. albigula. Three isolates of a novel arenavirus (proposed name ''Whitewater Arroyo,'' WWA) were recovered from two arenavirus antibody-positive N. albigula collected from whitewater Arroyo in McKinley County, New Mexico. Two-way serologic tests indicated that WWA virus is antigenically distinct from other arenaviruses but most closely related to TAM virus. Phylogenetic analysis of nucleocapsid protein gene sequence data showed that WWA virus is a novel arenavirus that is genetically most closely related to TAM virus, The recovery of WWA virus from antibody-positive N. albigula suggests that WWA virus infection in this species can be chronic and thus that N. albigula is a reservoir host of the virus. (C) 1996 Academic Press, Inc.