Cardiac Tissues From Stem Cells: New Routes to Maturation and Cardiac Regeneration.
Cardiac Tissues From Stem Cells: New Routes to Maturation and Cardiac Regeneration.
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DOI：
10.1161/circresaha.121.318183
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发表时间：
2021-03-19
影响因子：
20.1
通讯作者：
Mummery CL
中科院分区：
文献类型：
--
作者：
Campostrini G;Windt LM;van Meer BJ;Bellin M;Mummery CL
人类多能干细胞形成人体所有细胞的能力为研究疾病和产生可用于再生医学治疗的细胞提供了许多机会。尽管跳动的心肌细胞是最早从人类多能干细胞分化出来的细胞类型之一，但心脏应用的进展要比大脑、眼睛和胰腺的应用进展得慢。这在一定程度上是因为，简单的二维人类多能干细胞心肌细胞培养似乎需要在三维心脏结构中通常存在的关键功能线索。最近的组织工程方法结合对非心肌细胞和心肌细胞之间对话的新见解，解决了心肌细胞不成熟和无法概括成人心脏收缩、电生理或新陈代谢等特征的问题，并提供了解决方案。因此，三维生物工程心脏组织有望为疾病建模、药物发现和安全药理学做出重大贡献，并为心脏修复提供新的方式。在这里，我们回顾了三维工程化心脏组织的现状。
The ability of human pluripotent stem cells to form all cells of the body has provided many opportunities to study disease and produce cells that can be used for therapy in regenerative medicine. Even though beating cardiomyocytes were among the first cell types to be differentiated from human pluripotent stem cell, cardiac applications have advanced more slowly than those, for example, for the brain, eye, and pancreas. This is, in part, because simple 2-dimensional human pluripotent stem cell cardiomyocyte cultures appear to need crucial functional cues normally present in the 3-dimensional heart structure. Recent tissue engineering approaches combined with new insights into the dialogue between noncardiomyocytes and cardiomyocytes have addressed and provided solutions to issues such as cardiomyocyte immaturity and inability to recapitulate adult heart values for features like contraction force, electrophysiology, or metabolism. Three-dimensional bioengineered heart tissues are thus poised to contribute significantly to disease modeling, drug discovery, and safety pharmacology, as well as provide new modalities for heart repair. Here, we review the current status of 3-dimensional engineered heart tissues.