Rab11 is required during Drosophila eye development
Rab11 is required during Drosophila eye development
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DOI：
10.1387/ijdb.051986da
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发表时间：
2005-01-01
影响因子：
0.7
通讯作者：
Roy, JK
中科院分区：
文献类型：
--
作者：
Alone, DP;Tiwari, AK;Roy, JK
为了确定一个小的GTP结合蛋白Rab11在果蝇分化过程中的作用，研究了Rab11不同等位基因的表型表现。在不同的发育阶段，表现型从眼睛缺陷、硬毛异常、不育到致死。在这篇文章中，我们的重点是针对Rab17突变引起的眼部缺陷。Rab11(Mo)是一个新的P元件插入位点，其视网膜表现出特征性的异常，这种异常可以通过切除P元件和表达Rab11(+)转基因来逆转。在幼虫发育过程中，Rab11在光感受器细胞中被广泛合成，并定位于成虫眼睛的横纹肌和神经板。在Rab77(EP(3)3017)产生的纯合子克隆中，光感受器和相关的刷毛未能形成。Rab11(Mo)三龄幼虫眼触角视盘中Rab11蛋白水平的降低和细胞死亡的增加表明视网膜缺陷起源于幼虫的发育过程。我们的数据表明，在果蝇眼睛发育过程中，小眼的分化需要Rab11。
In an effort to identify the role of Rab11, a small GTP binding protein, during Drosophila differentiation, phenotypic manifestations associated with different alleles of Rab11 were studied. The phenotypes ranged from eye-defects, bristle abnormalities and sterility to lethality during various developmental stages. In this paper, our focus is targeted on eye defects caused by Rab17 mutations. A novel P-element insertion in the Rab11 locus, Rab11(mo), displayed characteristic retinal anomalies, which could be reverted by P-element excision and expression of Rab11(+) transgenes. During larval development, Rab11 is widely synthesized in photoreceptor cells and localizes to the rhabdomeres and lamina neuropil in adult eyes. Photoreceptors and associated bristles failed to be formed in homozygous clones generated in Rab77(EP(3)3017) eyes. Decreased levels of Rab11 protein and increased cell death in Rab11(mo) third-instar larval eye-antennal discs suggest that the retinal defects originate during larval development. Our data indicate a requirement for Rab11 in ommaticlial differentiation during Drosophila eye development.