Improved method for large-scale purification of brain gangliosides by Q-sepharose column chromatography. Immunochemical detection of C-series polysialogangliosides in adult bovine brains.
Improved method for large-scale purification of brain gangliosides by Q-sepharose column chromatography. Immunochemical detection of C-series polysialogangliosides in adult bovine brains.
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Q-琼脂糖柱层析大规模纯化脑神经节苷脂的改进方法。
DOI：
10.1016/s0021-9673(01)84550-7
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Susumu Ando
中科院分区：
文献类型：
--
作者：
Y. Hirabayashi;T. Nakao;M. Matsumoto;K. Obata;Susumu Ando
建立了一种用Q-Sepharose分离牛脑神经节苷的色谱方法。不仅根据它们的唾液酸数目，而且根据唾液酸分子种类和骨架寡糖部分的链长来分离神经节苷。以下结果表明，该柱色谱法在分离神经节苷类混合物，特别是位置异构体和具有n -乙酰基或n -糖基神经氨酸的分子种方面具有实际优势。(1) Q-Sepharose对神经节苷类的负载能力非常高；(2) GM1、GD1a、GD1b、GT1b、GQ1b等主要神经节苷类化合物在一次分离中分离得到；(3)使用Hanganutziu-Deicher抗体检测时，这些主要的神经节苷脂与含有n -糖基神经氨酸的神经节苷脂明显分离；(4)具有4个或4个以上唾液酸残基的聚唾液脂苷被有效分离。采用Q-Sepharose柱层析与薄层层析/酶免疫染色相结合的方法发现，成年牛脑中含有少量的c系列多涎神经节苷类成分，这些成分在鸟类和哺乳动物脑中被称为胚胎分子。
A new chromatographic method for separation of bovine brain gangliosides has been developed using Q-Sepharose. Gangliosides were separated based not only on their sialic acid numbers but also on the sialic acid molecular species and chain lengths of the skeletal oligosaccharide portions. The following results indicate that this column chromatography has practical advantages in separating mixtures of gangliosides, especially positional isomers and molecular species with N-acetyl- or N-glycolylneuraminic acid. (1) the loading capacity of Q-Sepharose for gangliosides was very high; (2) most major gangliosides such as GM1, GD1a, GD1b, GT1b and GQ1b were isolated in a single step; (3) these major gangliosides were clearly separated from gangliosides containing, N-glycolylneuraminic acid when examined using Hanganutziu-Deicher antibody; (4) polysialogangliosides that have four or more sialic acid residues were isolated efficiently. It was shown by the combination of Q-Sepharose column chromatography with thin-layer chromatography/enzyme immunostaining that adult bovine brains possess C-series polysialogangliosides as minor components which are known as embryonic molecules in avian and mammalian brains.
从 Tay-Sachs 大脑中分离和表征新型单唾液酸基五己糖基神经酰胺。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Itoh,T;Li,YT;Li,SC;Yu,RK
通讯作者：
Yu,RK