A rapid and simple microprocedure for myelin isolation.
A rapid and simple microprocedure for myelin isolation.
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一种快速、简单的髓磷脂分离微程序。
DOI：
10.1111/j.1471-4159.1980.tb04648.x
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发表时间：
1980
影响因子：
4.7
通讯作者：
Jones,M
中科院分区：
文献类型：
--
作者：
Keenan,RW;Jones,M
为了从大脑或脊髓的离散区域进行髓鞘的酶学研究，我们需要一种方法，能够从重量为50毫克或更少的组织样本中产生纯化的髓鞘。已经报道的微程序，如Detering和Wells(1976)或Fagg等人的程序。(1978)不适合快速和常规的髓鞘制备。在这次交流中，报告了我们能够从仅5毫克的整个大脑中分离出髓鞘的实验结果。数据表明，用这种方法分离的髓鞘可与Norton和Poduslo(1973a)的大尺度方法分离的髓鞘相媲美。
In order to conduct enzymatic studies on myelin from discrete regions of brain or spinal cord, we required a method capable of yielding purified myelin from tissue samples weighing 50 mg or less. Microprocedures that have been reported such as that of Detering and Wells (1976) or Fagg et al. (1978) were not suitable for rapid and routine myelin preparations. In this communication, the results of experiments in which we were able to isolate myelin from as little as 5 mg of whole brain are reported. Data are presented to show that the myelin isolated by this procedure is comparable to the myelin isolated by the macro‐scale procedure of Norton and Poduslo (1973a).