Intratumour Fusobacterium nucleatum and immune response to oesophageal cancer
Intratumour Fusobacterium nucleatum and immune response to oesophageal cancer
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DOI：
10.1038/s41416-022-02112-x
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发表时间：
2023-01-04
影响因子：
8.8
通讯作者：
Baba, Hideo
中科院分区：
文献类型：
--
作者：
Kosumi, Keisuke;Baba, Yoshifumi;Baba, Hideo
背景：实验证据表明，肿瘤内核梭杆菌通过下调抗肿瘤免疫参与胃肠道肿瘤的侵袭行为。我们研究了肿瘤内核梭菌与食管癌免疫应答之间的关系。方法:利用300例切除的食管癌的无偏数据库，我们使用定量聚合酶链反应法测量了肿瘤组织中具核梭菌的DNA，并评估了具核梭菌的丰度与T细胞密度（CD8 +， FOXP3 +和PDCD1 +）以及淋巴细胞反应模式（滤泡淋巴细胞反应，肿瘤周围淋巴细胞反应，肿瘤细胞反应）之间的关系。食管癌组织间质淋巴细胞反应及肿瘤浸润淋巴细胞。结果：具核梭菌仅与瘤周淋巴细胞反应呈显著负相关（P = 0.0002）。与无核梭菌组相比，高核梭菌组瘤周淋巴细胞反应明显降低（单变量优势比为0.33；95%可信区间为0.16-0.65;P = 0.0004）。多变量模型得出了类似的结果（多变量优势比为0.34；95%置信区间为0.16-0.69;P = 0.002）。结论：肿瘤内核仁梭菌与肿瘤周围淋巴细胞反应减弱有关，为进一步研究肿瘤内核仁梭菌与宿主免疫之间潜在的相互作用提供了平台。
Background: Experimental evidence suggests a role of intratumour Fusobacterium nucleatum in the aggressive behaviour of gastrointestinal cancer through downregulating anti-tumour immunity. We investigated the relationship between intratumour F. nucleatum and immune response to oesophageal cancer. Methods: Utilising an unbiased database of 300 resected oesophageal cancers, we measured F. nucleatum DNA in tumour tissue using a quantitative polymerase chain reaction assay, and evaluated the relationship between the abundance of F. nucleatum and the densities of T cells (CD8 + , FOXP3 + and PDCD1 + ), as well as lymphocytic reaction patterns (follicle lymphocytic reaction, peritumoural lymphocytic reaction, stromal lymphocytic reaction and tumour-infiltrating lymphocytes) in oesophageal carcinoma tissue. Results: F. nucleatum was significantly and inversely associated only with the peritumoural lymphocytic reaction (P = 0.0002). Compared with the F. nucleatum-absent group, the F. nucleatum-high group showed a much lower level of the peritumoural lymphocytic reaction (univariable odds ratio, 0.33; 95% confidence interval, 0.16-0.65; P = 0.0004). A multivariable model yielded a similar finding (multivariable odds ratio, 0.34; 95% confidence interval 0.16-0.69; P = 0.002). Conclusions: Intratumour F. nucleatum is associated with a diminished peritumoural lymphocytic reaction, providing a platform for further investigations on the potential interactive roles between intratumour F. nucleatum and host immunity.