Extrusion bioprinting: Recent progress, challenges, and future opportunities
Extrusion bioprinting: Recent progress, challenges, and future opportunities
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DOI：
10.1016/j.bprint.2020.e00116
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发表时间：
2021-03-01
期刊：
影响因子：
--
通讯作者：
Rivero, Iris, V
中科院分区：
文献类型：
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作者：
Ramesh, Srikanthan;Harrysson, Ola L. A.;Rivero, Iris, V
基于挤压的生物打印包括通过喷嘴挤压生物墨水来创建三维结构。生物链接包含与生物相关的新兴应用的生物体，如组织支架、器官芯片、再生医学和药物输送系统。生物墨水是生物材料和活细胞的混合物，通过其物理、机械、生物和流变行为影响打印结构的质量。可打印性是生物墨水的一种特性，用于描述其创建定义良好的结构的能力。在所有的影响因素中，流变性能和打印参数是影响生物打印结构质量的主要因素。随着挤出生物打印技术的日益普及，出现了不同的方法来控制这些性能和参数。本文重点介绍了流变学和工艺参数在挤出生物打印中的作用，并讨论了用于测量和定义生物油墨可打印性的定性和定量方法。最后，概述了挤出生物打印的主要挑战和未来趋势。
Extrusion-based bioprinting involves extrusion of bioinks through nozzles to create three-dimensional structures. The bioink contains living organisms with biological relevance for emerging applications such as tissue scaffolds, organs-on-a-chip, regenerative medicine, and drug delivery systems. Bioinks, which are mixtures of biomaterials and living cells, influence the quality of printed constructs through their physical, mechanical, biological, and rheological behavior. Printability is a property of a bioink used to describe its ability to create well-defined structures. Amongst all contributing factors, rheological properties and printing parameters are primary factors that influence the quality of bioprinted constructs. With the increasing popularity of extrusion bioprinting, different approaches for controlling these properties and parameters have emerged. This review highlights the role of rheology and process parameters in extrusion bioprinting and discusses qualitative and quantitative methods proposed to measure and define the printability of bioinks. Finally, an overview of key challenges and future trends in extrusion bioprinting is provided.