Efficient Reduction of Nitrobenzene to Aniline with a Biocatalyzed Cathode
Efficient Reduction of Nitrobenzene to Aniline with a Biocatalyzed Cathode
复制标题
利用生物催化阴极将硝基苯有效还原为苯胺
DOI：
10.1021/es202356w
复制
发表时间：
2011-12-01
影响因子：
11.4
通讯作者：
Rabaey, Korneel
中科院分区：
文献类型：
--
作者：
Wang, Ai-Jie;Cheng, Hao-Yi;Rabaey, Korneel
硝基苯（NB）是一种有毒化合物，在环境中经常被发现是一种污染物。目前的去除策略存在成本高或转化率慢的问题。在这里，我们研究了NB转化为苯胺（AN），这是一种毒性较小的最终产物，易于矿化，使用带有微生物催化阴极的进料式生物电化学系统。在葡萄糖存在的条件下，施加0.5 V电压，在24 h内，供试NB （0.5 mM）的88.2 +/- 0.60%转化为AN，分别比非生物阴极和开路控制实验高10.25倍和2.90倍。硝基苯是生物电化学还原NB过程中唯一检测到的产物（最高效率为98.70 +/- 0.87%），而在非生物条件下，亚硝基苯是NB还原AN的中间产物（效率降至73.75 +/- 3.2%）。当NaHCO3取代葡萄糖时，NB的降解率降低了约10%，但仍能实现NB向AN的选择性转化（98.93 +/- 0.77%）。阴极电极经高压灭菌后，硝基苯作为中间体形成，导致an形成效率下降71.6%。循环伏安法强调了更高的峰值电流以及生物阴极上NB还原的过电位降低。基于16S rRNA的阴极生物膜分析表明，阴极以一种与水生肠球菌密切相关的肠球菌为主。
Nitrobenzene (NB) is a toxic compound that is often found as a pollutant in the environment. The present removal strategies suffer from high cost or slow conversion rate. Here, we investigated the conversion of NB to aniline (AN), a less toxic endproduct that can easily be mineralized, using a fed-batch bioelectrochemical system with microbially catalyzed cathode. When a voltage of 0.5 V was applied in the presence of glucose, 88.2 +/- 0.60% of the supplied NB (0.5 mM) was transformed to AN within 24 h, which was 10.25 and 2.90 times higher than an abiotic cathode and open circuit controlled experiment, respectively. AN was the only product detected during bioelectrochemical reduction of NB (maximum efficiency 98.70 +/- 0.87%), whereas in abiotic conditions nitrosobenzene was observed as intermediate of NB reduction to AN (decreased efficiency to 73.75 +/- 3.2%). When glucose was replaced by NaHCO3, the rate of NB degradation decreased about 10%, selective transformation of NB to AN was still achieved (98.93 +/- 0.77%). Upon autoclaving the cathode electrode, nitrosobenzene was formed as an intermediate, leading to a decreased AN formation efficiency of 71.6%. Cyclic voltammetry highlighted higher peak currents as well as decreased overpotentials for NB reduction at the biocathode. 16S rRNA based analysis of the biofilm on the cathode indicated that the cathode was dominated by an Enterococcus species closely related to Enterococcus aquimarinus.