Folate receptor–targeted quantum dot liposomes as fluorescence probes
Folate receptor–targeted quantum dot liposomes as fluorescence probes
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DOI：
10.1080/10611860903013248
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发表时间：
2009-07
影响因子：
4.5
通讯作者：
Chang Yang;Nan Ding;Yibing Xu;Xiaolan Qu;Jing Zhang;Chao Zhao;Liang Hong;Yao Lu;Guangya Xia
中科院分区：
文献类型：
--
作者：
Chang Yang;Nan Ding;Yibing Xu;Xiaolan Qu;Jing Zhang;Chao Zhao;Liang Hong;Yao Lu;Guangya Xia
本研究报道了新型显像剂——包封亲水性CdTe量子点（QDs）的叶酸受体（FR）靶向脂质体的制备及其作为活细胞成像发光探针的应用。在水相中直接合成亲水性CdTe量子点，用CdTe悬浮液将脂质薄膜水化制备fr靶向量子点脂质体。通过紫外可见和荧光测量、脂质体粒径和zeta电位对制剂进行表征。研究fr靶向脂质体对人宫颈癌细胞株（HeLa）的靶向和成像能力。此外，通过与fr靶向QD脂质体、非靶向QD脂质体和游离QD孵育的HeLa细胞来评估QD脂质体的细胞毒性。结果表明，fr靶向QD脂质体呈球形，荧光收率高，光化学稳定性好，靶向性好，细胞毒性小。fr靶向荧光脂质体的平均粒径为~105 nm，粒径分布较窄。4°C保存11个月后，QD脂质体大小保持一致，未出现QD渗漏现象。fr靶向CdTe QD脂质体可以通过fr介导的内吞作用靶向肿瘤细胞，将成为一种有吸引力的肿瘤细胞或组织成像探针，可以长期监测肿瘤细胞或组织。
In this study, the preparation of the novel imaging agents, folate receptor (FR)–targeted liposomes encapsulating hydrophilic CdTe quantum dots (QDs), and their use as luminescence probes for live cell imaging are reported. Hydrophilic CdTe QDs were directly synthesized in the water phase, and FR-targeted QD liposomes were prepared by hydrating the lipid thin film with CdTe suspension. Formulations were characterized by UV-visible and fluorescent measurements, liposomal particle size, and zeta potential. The targeting and imaging ability of FR-targeted liposomes were investigated against the human uterine cervix cancer cell line (HeLa). Furthermore, the cytotoxicity of QD liposomes was evaluated by HeLa cells incubated with FR-targeted QD liposomes, nontargeted QD liposomes, and free QDs. The results showed that FR-targeted QD liposomes were spherically shaped with high fluorescence yield, excellent photochemical stability, good cancer targeting, and minimal cytotoxicity. The average size of FR-targeted fluorescence liposomes was ~105 nm, and their size distribution was rather narrow. After storage at 4°C for 11 months, QD liposomes maintained similar size and did not show any leakage of QDs. FR-targeted CdTe QD liposomes, which can target tumor cells via FR-mediated endocytosis, would become an attractive probe for tumor cell or tissue imaging for a long-time monitoring.