Differential Changes in Responses of Hypothalamic and Brainstem Neuronal Populations to Prolactin During Lactation in the Mouse
Differential Changes in Responses of Hypothalamic and Brainstem Neuronal Populations to Prolactin During Lactation in the Mouse
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DOI：
10.1095/biolreprod.110.089185
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发表时间：
2011-04-01
影响因子：
3.6
通讯作者：
Grattan, D. R.
中科院分区：
文献类型：
--
作者：
Brown, R. S. E.;Herbison, A. E.;Grattan, D. R.
在哺乳期间，母亲的大脑有许多功能上的适应。有证据表明，哺乳期间血液中的高水平催乳素可能有助于这些适应性变化。本研究以催乳素诱导的信号转导和转录激活子5(PSTAT5)磷酸化为指标，研究哺乳期小鼠脑内催乳素介导的功能性信号转导水平，并与发情间期外源性催乳素的作用进行比较。在哺乳第7天，在小鼠前脑和脑干的许多区域观察到广泛的pSTAT5的诱导。在前脑的视前内侧核、终纹床核、室旁核和杏仁内侧核，以及在脑干的中脑导水管周围灰质、臂旁核、中缝背侧和中缝隐核，与发情间期外源性催乳素的反应相比，哺乳期间pSTAT5的表达显著增加。在前腹侧脑室周围核、弓状核、腹内侧核和背内侧核，哺乳期的反应类似于发情间期。相反，在脑干的最后区域，在哺乳期间，对催乳素的反应减少，pSTAT5表达丢失。通过原位杂交检测，无论是急性还是慢性催乳素升高，这些不同的反应都没有伴随着催乳素受体mRNA水平的任何变化。这些数据与催乳素可能在母体大脑中调节广泛的适应性反应的假设一致。
During lactation, there are numerous functional adaptations in the maternal brain. There is evidence that the high levels of circulating prolactin present during lactation might contribute to these adaptive changes. The present study aimed to investigate levels of functional prolactin-mediated signal transduction in the brain of lactating mice, using prolactin-induced phosphorylation of signal transducer and activator of transcription 5 (pSTAT5) as a marker, and compare these to the effect of exogenous prolactin during diestrus. On Day 7 of lactation, widespread induction of pSTAT5 was observed in numerous regions of the mouse forebrain and brainstem. In the medial preoptic nucleus, bed nuclei stria terminalis, paraventricular nucleus, and medial amygdala of the forebrain, and in the rostral periaqueductal gray, parabrachial nucleus, dorsal raphe, and the raphe obscurus nucleus of the brainstem, pSTAT5 expression was markedly increased during lactation compared with the response to exogenous prolactin during diestrus. In the anteroventral periventricular nucleus, arcuate nucleus, ventromedial nucleus, and dorsomedial nucleus, responses in lactation were comparable to diestrus. Conversely, in the area postrema of the brainstem, there was a reduction in response to prolactin, with a loss of pSTAT5 expression, during lactation. These differential responses following either acute or chronic elevations in prolactin were not accompanied by any changes in levels of prolactin receptor mRNA, when measured by in situ hybridization. These data are consistent with the hypothesis that prolactin might mediate widespread adaptive responses in the maternal brain.