Role of a cysteine synthase in Staphylococcus aureus
Role of a cysteine synthase in Staphylococcus aureus
复制标题
DOI：
10.1128/jb.186.6.1579-1590.2004
复制
发表时间：
2004-03-01
影响因子：
3.2
通讯作者：
Foster, SJ
中科院分区：
文献类型：
--
作者：
Lithgow, JK;Hayhurst, EJ;Foster, SJ
革兰氏阳性的人类病原体金黄色葡萄球菌经常用含有亚碲酸钾的培养基分离，它比大肠杆菌具有更高水平的抗性。色葡萄aureus cysM基因是在筛选基因中分离的，所述基因将增加E. coli DH5 α。S.金黄色葡萄球菌cysM与半胱氨酸合酶蛋白的O-乙酰丝氨酸（巯基）-裂解酶B家族在序列和功能上同源。色葡萄金黄色葡萄球菌cysM敲除突变体在半胱氨酸限制条件下生长不良，并且细胞提取物中巯基含量的分析表明，cysM突变体产生的半胱氨酸显著少于野生型S.金黄色葡萄球菌SH 1000。S.金黄色葡萄球菌SH 1000不能使用硫酸盐、亚硫酸盐或磺酸盐作为半胱氨酸生物合成中的硫源，这可以通过在金黄色葡萄球菌中缺乏摄取和还原这些化合物所需的基因来解释。金黄色葡萄球菌基因组。S.然而，金黄色葡萄球菌SH 1000可以利用硫代硫酸盐、硫化物或谷胱甘肽作为硫的唯一来源。cysM基因突变导致S.金黄色葡萄球菌对亚碲酸盐、过氧化氢、酸和二酰胺的耐受能力也显著降低。金黄色葡萄球菌恢复饥饿的氨基酸或磷酸盐限制条件下，表明半胱氨酸在S。金黄色葡萄球菌应激反应和生存机制。
The gram-positive human pathogen Staphylococcus aureus is often isolated with media containing potassium tellurite, to which I it has a higher level of resistance than Escherichia coli. The S. aureus cysM gene was isolated in a screen for genes that would increase the level of tellurite resistance of E. coli DH5alpha. The protein encoded by S. aureus cysM is sequentially and functionally homologous to the O-acetylserine (thiol)-lyase B family of cysteine synthase proteins. An S. aureus cysM knockout mutant grows poorly in cysteine-limiting conditions, and analysis of the thiol content in cell extracts showed that the cysM mutant produced significantly less cysteine than wild-type S. aureus SH1000. S. aureus SH1000 cannot use sulfate, sulfite, or sulfonates as the source of sulfur in cysteine biosynthesis, which is explained by the absence of genes required for the uptake and reduction of these compounds in the S. aureus genome. S. aureus SH1000, however, can utilize thiosulfate, sulfide, or glutathione as the sole source of sulfur. Mutation of cysM caused increased sensitivity of S. aureus to tellurite, hydrogen peroxide, acid, and diamide and also significantly reduced the ability of S. aureus to recover from starvation in amino acid- or phosphate-limiting conditions, indicating a role for cysteine in the S. aureus stress response and survival mechanisms.