The spatial regulation of condensin activity in chromosome condensation.
The spatial regulation of condensin activity in chromosome condensation.
复制标题
染色体凝缩中凝缩蛋白活性的空间调控。
DOI：
10.1101/gad.335471.119
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发表时间：
2020
影响因子：
10.5
通讯作者：
Koshland,DouglasE
中科院分区：
文献类型：
--
作者：
Lamothe,Rebecca;Costantino,Lorenzo;Koshland,DouglasE
凝聚素介导染色体凝聚，这是有丝分裂过程中染色体正确分离所必需的。在芽殖酵母的后期之前，核糖体DNA（RDN）浓缩成与其余染色体不同的薄环。我们提供的证据表明，这种RDN缩合的建立和维护需要Cdc5p（波罗）激酶调节缩合素。我们发现，Cdc5p被招募到凝聚素结合的RDN中的凝聚素，一个复杂的凝聚素相关的网站。Cdc5p和cohesin防止凝聚蛋白将RDN错误折叠成不可逆的解凝聚状态。从这些和其他的观察，我们建议，空间调节Cdc5p的凝聚调节凝聚活性，以确保正确的RDN折叠成一个薄的循环。这种机制可能是进化上保守的，促进了植物和动物有丝分裂染色体的着丝粒和RDN处的稀疏压缩。
Condensin mediates chromosome condensation, which is essential for proper chromosome segregation during mitosis. Prior to anaphase of budding yeast, the ribosomal DNA (RDN) condenses to a thin loop that is distinct from the rest of the chromosomes. We provide evidence that the establishment and maintenance of this RDN condensation requires the regulation of condensin by Cdc5p (polo) kinase. We show that Cdc5p is recruited to the site of condensin binding in the RDN by cohesin, a complex related to condensin. Cdc5p and cohesin prevent condensin from misfolding the RDN into an irreversibly decondensed state. From these and other observations, we propose that the spatial regulation of Cdc5p by cohesin modulates condensin activity to ensure proper RDN folding into a thin loop. This mechanism may be evolutionarily conserved, promoting the thinly condensed constrictions that occur at centromeres and RDN of mitotic chromosomes in plants and animals.