Accuracy of motor axon regeneration across autograft, single-lumen, and multichannel poly(lactic-co-glycolic acid) nerve tubes
Accuracy of motor axon regeneration across autograft, single-lumen, and multichannel poly(lactic-co-glycolic acid) nerve tubes
复制标题
DOI：
10.1227/01.neu.0000335081.47352.78
复制
发表时间：
2008-07-01
期刊：
影响因子：
4.8
通讯作者：
Windebank, Anthony J.
中科院分区：
文献类型：
--
作者：
de Ruiter, Godard C.;Spinner, Robert J.;Windebank, Anthony J.
目的：运动神经轴突再生的准确性成为发展用于运动神经修复的神经管的重要问题。神经管再生的分散可能导致错误的方向和多神经支配。在这项研究中，我们提出了一系列的方法来调查再生的准确性，我们用来比较再生跨自体移植物和单腔聚（乳酸-共-乙醇酸）（PLGA）神经管。我们还提出了多通道神经管的概念，可能会限制分散分别指导组的再生axons.METHODS：的同时跟踪的胫神经和腓神经与快蓝和diamidino黄8周后，修复1厘米的神经间隙，在大鼠坐骨神经，以确定双投射运动神经元的百分比。修复前1周用二脒基黄和修复后8周用固蓝连续追踪腓神经，以确定腓神经运动神经元正确定向的百分比。在单腔神经管成功再生的病例中，单腔神经管修复后，更多的运动神经元向胫神经和腓神经分支双投射（21.4%）高于自体移植修复后（5.9%）。多通道神经管修复后，这一百分比略有下降（16.9%），但不显著。结论：逆行追踪技术为神经管再生过程提供了新的视角。本研究提供的方法和数据可作为开发用于运动神经修复的神经管的基础。
OBJECTIVE: The accuracy of motor axon regeneration becomes an important issue in the development of a nerve tube for motor nerve repair. Dispersion of regeneration across the nerve tube may lead to misdirection and polyinnervation. In this study, we present a series of methods to investigate the accuracy of regeneration, which we used to compare regeneration across autografts and single-lumen poly(lactic-co-glycolic acid) (PLGA) nerve tubes. We also present the concept of the multichannel nerve tube that may limit dispersion by separately guiding groups of regenerating axons.METHODS: The simultaneous tracing of the tibial and peroneal nerves with fast blue and diamidino yellow was performed 8 weeks after the repair of a 1-cm nerve gap in the rat sciatic nerve to determine the percentage of double-projecting motoneurons. Sequential tracing of the peroneal nerve with diamidino yellow 1 week before repair and fast blue 8 weeks after repair was performed to determine the percentage of correctly directed peroneal motoneurons.RESULTS: In the cases in which there was successful regeneration across single-lumen nerve tubes, more motoneurons had double projections to both the tibial and peroneal nerve branches after single-lumen nerve tube repair (21.4%) than after autograft repair (5.9%). After multichannel nerve tube repair, this percentage was slightly reduced (16.9%), although not significantly. The direction of regeneration was nonspecific after all types of repair.CONCLUSION: Retrograde tracing techniques provide new insights into the process of regeneration across nerve tubes. The methods and data presented in this study can be used as a basis for the development of a nerve tube for motor nerve repair.